Lysozyme in preosseous cartilage VII. Evidence for physiological role of losozyme in normal endochondral calcification
Lysozyme in preosseous cartilage VII. Evidence for physiological role of losozyme in normal endochondral calcification
复制标题
骨前软骨中的溶菌酶 VII。
DOI：
10.1016/0304-4165(74)90195-0
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发表时间：
1974
期刊：
影响因子：
--
通讯作者：
J. C. Pita
中科院分区：
文献类型：
--
作者：
K. Kuettner;N. Sorgente;R. Croxen;D. Howell;J. C. Pita
以前的工作表明，从大鼠表观软骨板的微穿刺吸出物含有成核剂的钙（PO 4）2矿物生长，和成核抑制蛋白多糖聚集体。在本报告中描述的数据表明，哺乳动物溶菌酶使抑制失活。当微穿刺吸出物与哺乳动物溶菌酶在体外孵育时，发生快速、自发的矿物质生长起始。在软骨溶菌酶而不是鸡蛋白色溶菌酶存在下孵育蛋白聚糖聚集体制剂，导致蛋白聚糖的沉降系数显著降低，通常接近于用蛋白聚糖单体制剂获得的值。通过酶三（N-乙酰基-D-葡糖胺）的特异性抑制剂抑制哺乳动物溶菌酶的这种作用，表明它可能是酶性质的。
Previous work demonstrated that micropuncture aspirates from rat epiphysical plate cartilage contain a nucleating agent for Ca3(PO4)2mineral growth, and that the nucleation is inhibited by proteoglycan aggregates. In this report data are described which show that mammalian lysozyme inactivates the inhibition. When micropuncture aspirates are incubated in vitro with mammalian lysozyme, a rapid, spontaneous initiation of mineral growth occurs. Incubation of proteoglycan aggregate preparations in the presence of cartilagea lysozyme, but not hen egg white lysozyme, causes a marked decrease of the sedimentation coefficients of the proteoglycans, usually to values close to those obtained with proteoglycan monomer preparations. The inhibition of this effect of mammalian lysozyme by a specific inhibitor of the enzyme tri(N-acetyl-D-glucosamine) suggests that it may be enzymatic in nature.