Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase.
Studies on acetylcholine sensor and its analytical application based on the inhibition of cholinesterase.
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DOI：
10.1016/0956-5663(90)80035-c
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发表时间：
1990
影响因子：
12.6
通讯作者：
C. Tran‐Minh;P. Pandey;S. Kumaran
中科院分区：
文献类型：
--
作者：
C. Tran‐Minh;P. Pandey;S. Kumaran
Acetylcholine esterase electrodes, based on glass, Pd/PdO and Ir/IrO2electrodes as pH sensor, using the immobilized acetylcholine esterase in acrylamide-methacrylamide hydrazides prepolymer are reported and compared. New data on the analysis of nicotine, fluoride ion, and some organophosphorus compounds are reported using the present AChE sensor based on the inhibition of the immobilized acetylcholine esterase. Reactivation of immobilized AChE after inhibition with reversible inhibitor, i.e. nicotine and fluoride ion is carried out using a mixture of working buffer and acetylcholine, whereas reactivation after inhibition with irreversible inhibitor, i.e. organophosphorus compounds is carried out using a mixture of acetylcholine and pyridine-2-aldoxime methiodide (PAM). The detection limits for the nicotine and fluoride ion are found to be 10−5M whereas for paraoxon, methyl parathion and malathion are found to be 10−9M and 10−10M.
