Blood culture and polymerase chain reaction for the diagnosis of the chronic phase of human infection with Trypanosoma cruzi
Blood culture and polymerase chain reaction for the diagnosis of the chronic phase of human infection with Trypanosoma cruzi
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DOI：
10.1007/s00436-002-0679-3
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发表时间：
2002-10-01
影响因子：
2
通讯作者：
Galvao, LMC
中科院分区：
文献类型：
--
作者：
Castro, AM;Luquetti, AO;Galvao, LMC
在本研究中，我们首次评估了未经治疗的慢性恰加斯病的血液寄生特征。该研究在60名患者和9名血清学阴性个体的对照组中进行。3份血样分析，血培养累计阳性70%，PCR累计阳性86.7%。两种检测方法比较，41.1%（74/180）的标本PCR和血培养均阳性，22.2%（40/180）的标本PCR单独阳性，4.4%（8/180）的标本血培养阳性，PCR阴性。第二份样本的加入显著提高了血培养（P=0.0000）和PCR （P=0.0369）的阳性。第三个样本的加入对血培养也有统计学意义（P=0.0001），但对PCR没有统计学意义（P=0.1186）。这些数据表明，在进行特异性治疗之前，研究克氏锥虫感染个体的寄生虫病非常重要。他们还建议，至少应采集两份血液样本，并应采用两种检测方法，如果可能的话，采用一种能大大提高恰加斯病的寄生虫学诊断和治疗效果评价的方法。
In the present study, we evaluated for the first time the profile of blood parasitism in untreated, chronic Chagas' disease. The study was conducted on 60 patients and a control group of nine serologically negative individuals. Analysis of three blood samples showed 70% cumulative positivity for blood culture and 86.7% positivity for PCR. The comparison of the two tests revealed that 41.1% (74/180) of the samples presented positive results for both PCR and blood culture, 22.2% (40/180) were positive for PCR alone, and 4.4% (8/180) were positive for blood culture and negative for PCR. The addition of the second sample raised positivity significantly for both blood culture (P=0.0000) and PCR (P=0.0369). Addition of the third sample was also statistically significant for blood culture (P=0.0001) but not for PCR (P=0.1186). These data point to the importance of studying the parasitemia of Trypanosoma cruzi-infected individuals before specific treatment. They also suggest that at least two blood samples should be collected and that two tests should be used, if possible a procedure that considerably improves the parasitologic diagnosis of Chagas' disease and the evaluation of therapeutic efficacy.