DETECTING BENZIMIDAZOLE RESISTANCE WITH FECAL EGG COUNT REDUCTION TESTS AND INVITRO ASSAYS
DETECTING BENZIMIDAZOLE RESISTANCE WITH FECAL EGG COUNT REDUCTION TESTS AND INVITRO ASSAYS
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DOI：
10.1111/j.1751-0813.1989.tb13578.x
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发表时间：
1989-08-01
影响因子：
1.1
通讯作者：
JARRETT, RG
中科院分区：
文献类型：
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作者：
MARTIN, PJ;ANDERSON, N;JARRETT, RG
制备了由0、1、10、25、50、75、90和100%的已知抗药性菌株组成的croubriformis毛圆线虫和Osteragia spp的复合菌株，并通过粪卵减少试验、体外孵化试验和微管蛋白结合试验检测了苯并咪唑的抗性。当耐药菌株在复合材料中的比例为50%或更高时，所有测试都检测到抗药性，而没有一项测试明确检测到低于25%的抗药性。卵数减少试验在检测低水平抗性方面的敏感性不低于体外试验，但卵孵化和微管蛋白结合试验提供了更好的中到高度抗性水平的定量估计。因此，粪卵计数的减少提供了一种在野外检测抗药性的合适手段，但需要对低水平的抗药性更敏感的测试。结果表明，仅使用治疗后计数就足以说明驱虫效果。
Composite strains of Trichostrongylus colubriformis and Ostertagia spp consisting of 0,1,10,25,50,75,90, and 100% of known resistant strains were prepared and tested for benzimidazole resistance using faecal egg count reduction tests, in vitro egg hatch assays and tubulin binding assays. All tests detected resistance where the proportion of the resistant strain in the composite was 50% or more, whereas none of the tests unequivocally detected resistance below 25%. Egg count reduction tests were no less sensitive than the in vitro tests in detecting low levels of resistance but the egg hatch and tubulin binding assays provided a better quantitative estimate of moderate to high levels of resistance. Faecal egg count reduction therefore, provides a suitable means of detecting resistance in the field but tests, more sensitive to low levels of resistance are required. Results indicate that the use of post-treatment counts alone provides an adequate indication of anthelmintic efficiency.