The levels of hormone and carbohydrate in autumn and non-autumn flowering tree peonies
The levels of hormone and carbohydrate in autumn and non-autumn flowering tree peonies
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DOI：
10.1139/cjps2011-043
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发表时间：
2011-11
影响因子：
1.2
通讯作者：
P. M. P. Mornya-P.-M.-P.-Mornya-87649655;F. Cheng
中科院分区：
文献类型：
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作者：
P. M. P. Mornya-P.-M.-P.-Mornya-87649655;F. Cheng
Mornya，P. M. P.和Cheng，F. 2011.秋花与非秋花牡丹体内激素和碳水化合物含量的比较。可以的.植物科学杂志91：991-998。本研究采用高效液相色谱法分析了诱导过程中赤霉酸（GA 3）、吲哚乙酸（IAA）、细胞分裂素（CTK）和脱落酸（阿坝）等激素的含量，采用分光光度计分析了诱导过程中蔗糖、还原糖和淀粉含量，秋花牡丹品种和非秋花牡丹品种在开花格局上存在差异。实验设计为随机完全区组，重复三次。AFP和NAFP开花模式的差异很大程度上受不同芽发育时期GA 3、IAA和CTK水平差异的影响。花形成周期在AFP比在NAFP早完成，因此开花是每年两次。细胞分裂素，尤其是N6-（N2-异戊烯基）-腺苷（iPA），在牡丹秋季开花过程中可能起着关键作用，因为其在不同芽发育阶段的AFP和NAFP水平差异显著。然而，只有GA 3在芽发育阶段有负差异，表明GA 3水平始终高于NAFP比AFP，但CTK的情况正好相反。AFP和NAFP的GA_3、IAA和CTK含量至少在2/3的芽发育阶段差异显著（P<0.05）。碳水化合物可能不会显着影响牡丹的开花模式。因此，牡丹秋花期可通过调控GA 3、IAA和CTK水平来实现，尤其是在芽发育的诱导期和起始期，以促进花形成周期的完成，使其提前于芽休眠期。本研究结果可为牡丹开花规律的基因工程研究奠定科学基础。
Mornya, P. M. P. and Cheng, F. 2011. The levels of hormone and carbohydrate in autumn and non-autumn flowering tree peonies. Can. J. Plant Sci. 91: 991-998. This study analyzed the levels of gibberellic acid (GA3), indole-3-acetic acid (IAA), cytokinin (CTK) and abscisic acid (ABA) hormones using high-performance liquid chromatography, along with the levels of sucrose, reducing sugar and starch carbohydrates by spectrophotometer during induction, initiation and differentiation stages of bud development in autumn (AFP) and non-autumn (NAFP) flowering tree peony cultivars exhibiting variations in flowering pattern. The experimental design was a randomized complete block with three replications. The variation in flowering pattern between AFP and NAFP was largely influenced by differences in GA3, IAA and CTK levels at different bud developmental stages. The flower formation cycle was completed earlier in AFP than in NAFP, hence flowering was twice annually. Cytokinin, particularly N6-(▵2-isopentenyl)-adenosine (iPA), could be a critical hormone in autumn flowering of tree peony, as its differences in levels between AFP and NAFP remained significant across bud developmental stages. However, only GA3 had negative differences across bud developmental stages, indicating that GA3 levels were consistently higher in NAFP than in AFP, but the reverse holds true for CTK. The differences in GA3, IAA and CTK between AFP and NAFP were significant (P<0.05) for at least two-thirds of the bud developmental stages. Carbohydrates may not significantly influence the flowering pattern of tree peonies. Autumn flowering in tree peonies could therefore be achieved by regulating GA3, IAA and CTK levels, particularly at the induction and initiation stages of bud development to facilitate the completion of the floral formation cycle, well in advance of bud dormancy period. The findings of this study could lay the scientific basis for genetic engineering of flowering pattern of tree peonies.