Improved molecular technique for the differentiation of neotropical anopheline species
Improved molecular technique for the differentiation of neotropical anopheline species
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DOI：
10.4269/ajtmh.2008.78.492
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发表时间：
2008-03-01
影响因子：
3.3
通讯作者：
Kosek, Margaret
中科院分区：
文献类型：
--
作者：
Matson, Ryan;Rios, Carlos Tong;Kosek, Margaret
我们评估了PCR-RFLP的核糖体内转录间隔区2区（ITS2），以区分种按蚊在亚马逊地区的报告，并验证了这种方法使用饲养的F，后代。使用以下按蚊物种进行分子分析：（纽约州）benarrochi，安.（纽约州）darlingi，安.（纽约州）努涅兹托瓦里湾（纽约州）konderi，安.（纽约州）rangeli和An.（纽约州）广义triannulatus lato（s.l.）此外，还报道了按蚊亚属Anopheles，An.（Ano.）forattini，安.（Ano.）mattogrossensis和安氏隐翅虫An.（Ano.）包括Peryassui用于测试。每一个测试的9个物种产生诊断带型。的PCR-RFLP方法是成功的，在确定所有的生命阶段，包括蜕皮与小部分的样品。该测定是快速的，可以作为一个公正的确认方法，用于鉴定形态变异，有争议的样品，保存不完善的标本，和生命阶段的分类关键不允许明确的物种确定。
We evaluated a PCR-RFLP of the ribosomal internal transcribed spacer 2 region (ITS2) to distinguish species of Anopheles commonly reported in the Amazon and validated this method using reared F, offspring. The following species of Anopheles were used for molecular analysis: An. (Nys.) benarrochi, An. (Nys.) darlingi, An. (Nys.) nuneztovari, An. (Nys.) konderi, An. (Nys.) rangeli, and An. (Nys.) triannulatus sensu lato (s.l.). In addition, three species of the subgenus Anopheles, An. (Ano.) forattini, An. (Ano.) mattogrossensis, and An. (Ano.) peryassui were included for testing. Each of the nine species tested yielded diagnostic banding patterns. The PCR-RFLP method was successful in identifying all life stages including exuviae with small fractions of the sample. The assay is rapid and can be applied as an unbiased confirmatory method for identification of morphologic variants, disputed samples, imperfectly preserved specimens, and life stages from which taxonomic keys do not allow for definitive species determination.