Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening
Differential expression and internal feedback regulation of 1-aminocyclopropane-1-carboxylate synthase, 1-aminocyclopropane-1-carboxylate oxidase, and ethylene receptor genes in tomato fruit during development and ripening
复制标题
DOI：
10.1104/pp.118.4.1295
复制
发表时间：
1998-12-01
期刊：
影响因子：
7.4
通讯作者：
Inaba, A
中科院分区：
文献类型：
--
作者：
Nakatsuka, A;Murachi, S;Inaba, A
我们研究了番茄 (Lycopersicon esculentum) 果实乙烯生物合成从系统 1 到系统 2 乙烯生产转变的反馈调节。随着乙烯产量的激增，成熟果实中 LE-ACS2、LE-ACS 1 和 NR mRNA 的丰度增加。 1-甲基环丙烯（MCP）（一种乙烯作用抑制剂）处理可以在很大程度上阻止成熟过程中 mRNA 的增加。 LE-ACS6 基因的转录本在更年期前的果实中积累，但在未处理的成熟果实中不积累，但在用 MCP 处理的成熟果实中确实积累。用丙烯处理幼果可防止该基因转录物的积累。 LE-ACS1A、LE-ACS3 和 TAE1 基因在整个发育和成熟过程中在果实中组成型表达，无论果实是否用 MCP 还是丙烯处理。 LE-ACO1和LE-ACO4基因的转录本已经存在于跃变前的果实中，并且在成熟开始时大大增加。 MCP 处理也可以防止 LE-ACO mRNA 随着成熟而增加。结果表明，在番茄果实中，更年期前系统 1 乙烯可能是通过组成型表达的 LE-ACS1A 和 LE-ACS3 以及负反馈调节的 LE-ACS6 基因与预先存在的 LE-ACO1 和 LE-ACO4 mRNA 介导的。在更年期开始时，它转变为乙烯系统 2，由于正反馈调节，LE-ACS2、LE-ACS4、LE-ACO1 和 LE-ACO4 mRNA 大量积累。从系统 1 到系统 2 乙烯生产的转变可能与 NR mRNA 的积累水平有关。
We investigated the feedback regulation of ethylene biosynthesis in tomato (Lycopersicon esculentum) fruit with respect to the transition from system 1 to system 2 ethylene production. The abundance of LE-ACS2, LE-ACS 1, and NR mRNAs increased in the ripening fruit concomitant with a burst in ethylene production. These increases in mRNAs with ripening were prevented to a large extent by treatment with 1-methylcyclopropene (MCP), an ethylene action inhibitor. Transcripts for the LE-ACS6 gene, which accumulated in preclimacteric fruit but not in untreated ripening fruit, did accumulate in ripening fruit treated with MCP. Treatment of young fruit with propylene prevented the accumulation of transcripts for this gene. LE-ACS1A, LE-ACS3, and TAE1 genes were expressed constitutively in the fruit throughout development and ripening irrespective of whether the fruit was treated with MCP or propylene. The transcripts for LE-ACO1 and LE-ACO4 genes already existed in preclimacteric fruit and increased greatly when ripening commenced. These increases in LE-ACO mRNA with ripening were also prevented by treatment with MCP. The results suggest that in tomato fruit the preclimacteric system 1 ethylene is possibly mediated via constitutively expressed LE-ACS1A and LE-ACS3 and negatively feedback-regulated LE-ACS6 genes with preexisting LE-ACO1 and LE-ACO4 mRNAs. At the onset of the climacteric stage, it shifts to system 2 ethylene, with a large accumulation of LE-ACS2, LE-ACS4, LE-ACO1, and LE-ACO4 mRNAs as a result of a positive feedback regulation. This transition from system 1 to system 2 ethylene production might be related to the accumulated level of NR mRNA.