Postmortem accumulation of tubulin in postsynaptic density preparations.
Postmortem accumulation of tubulin in postsynaptic density preparations.
复制标题
死后突触后密度制剂中微管蛋白的积累。
DOI：
10.1111/j.1471-4159.1982.tb10858.x
复制
发表时间：
1982
影响因子：
4.7
通讯作者：
Siekevitz,P
中科院分区：
文献类型：
--
作者：
Carlin,RK;Grab,DJ;Siekevitz,P
从犬大脑皮质分离的突触后密度(PSD)制剂在0-37℃放置不同时间后，发现两条带的突触后密度(PSD)以时间依赖的方式丰富，而不是温度依赖的方式。用凝胶迁移率和免疫学方法鉴定这两条带为微管蛋白亚基。在所有孤立的突触结构中，微管蛋白的增加主要发生在PSD部分。犬小脑和大鼠前脑的PSD制备物中也有微管蛋白的增加。在动物死亡后尽快从犬脑中分离PSD组分的结果表明，通过凝胶电泳图扫描确定PSD制剂中微管蛋白的最大含量是总考马斯亮蓝蛋白的2.5%。这些结果表明微管蛋白可能不是PSD的主要结构蛋白，因为它存在于原位。
Postsynaptic density (PSD) preparations isolated from canine cerebral cortex that had been left at 0–37°C for various times were found to become enriched in two bands in a time‐ but not temperature‐dependent manner. The two bands were identified as tubulin subunits by gel mobility and immunology. Of all the isolated synaptic structures the increase in tubulin occurred primarily in the PSD fraction. The increase of tubulin also occurred in PSD preparations isolated from canine cerebellum and rat forebrain. Results obtained when PSD fractions were isolated from canine brain obtained as rapidly as possible after the death of the animal indicate that the maximum amount of tubulin in the PSD preparations is 2.5% of total Coomassie blue‐stained protein as determined by scanning of gel electrophoretograms. These results imply that tubulin is probably not a major structural protein of the PSD as it existsin situ.