Single-particle tracking: The distribution of diffusion coefficients
Single-particle tracking: The distribution of diffusion coefficients
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DOI：
10.1016/s0006-3495(97)78820-9
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发表时间：
1997-04-01
影响因子：
3.4
通讯作者：
Saxton, MJ
中科院分区：
文献类型：
--
作者：
Saxton, MJ
在单粒子示踪实验中，扩散系数D可以从细胞膜中单个粒子的轨迹来测量。用蒙特卡罗方法计算了单轨扩散系数的统计分布。这种分布的宽度可以用来衡量膜的非均质性，也可以用来检验膜中的受阻扩散模型。对于某些模型，短程扩散系数的分布比观察到的蛋白质在细胞膜中扩散的分布要窄得多。为了帮助分析单粒子跟踪测量，对不同的D定义和不同的轨迹长度检查了D的分布。
In single-particle tracking experiments, the diffusion coefficient D may be measured from the trajectory of an individual particle in the cell membrane. The statistical distribution of single-trajectory diffusion coefficients is examined by Monte Carlo calculations. The width of this distribution may be useful as a measure of the heterogeneity of the membrane and as a test of models of hindered diffusion in the membrane. For some models, the distribution of the short-range diffusion coefficient is much narrower than the observed distribution for proteins diffusing in cell membranes. To aid in the analysis of single-particle tracking measurements, the distribution of D is examined for various definitions of D and for various trajectory lengths.