DISTRIBUTION OF GABAERGIC AND CHOLINERGIC NEURONS IN THE RAT DIAGONAL BAND
DISTRIBUTION OF GABAERGIC AND CHOLINERGIC NEURONS IN THE RAT DIAGONAL BAND
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DOI：
10.1016/0306-4522(86)90258-7
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发表时间：
1986-02-01
期刊：
影响因子：
3.3
通讯作者：
HEIMER, L
中科院分区：
文献类型：
--
作者：
BRASHEAR, HR;ZABORSZKY, L;HEIMER, L
内侧隔-斜角带复合体中GABA能神经元与胆碱能神经元同延。连续切片，连续染色和双重免疫荧光技术采用抗体谷氨酸脱羧酶和胆碱乙酰转移酶揭示了这些发射特异性神经元在大鼠的分布。在形态学上，这两种类型的神经元似乎相似，因为它们主要是大型多极细胞，但它们的特点是不同的，在对角线上重叠分布。谷氨酸脱羧酶阳性细胞分散在整个细胞核的垂直肢体的斜角带，而胆碱乙酰转移酶阳性神经元是更多的medially和分布在相应的背侧和腹侧方面的核。在对角带水平肢核的吻侧部分，胆碱乙酰转移酶阳性细胞倾向于位于内侧，而尾侧，它们向核的背侧扩散，与腹侧苍白球和豆状核下无名质中的其他大型胆碱能神经元连续。绝大多数的谷氨酸脱羧酶阳性神经元保持在一个更腹侧和横向的位置内的核的水平肢，特别是众多的只是横向的斜角带纤维，因为它们加入内侧前脑束。据估计，胆碱能神经元的数量大约是GABA能神经元的两倍。大约1%的胆碱乙酰转移酶阳性神经元也谷氨酸脱羧酶阳性的双重免疫荧光研究，但不是在连续染色或连续切片。
GABAergic neurons are coextensive with cholinergic neurons in the medial septum-diagonal band complex. Serial sectioning, sequential staining and double immunofluorescence techniques employing antibodies to glutamate decarboxylase and choline acetyltransferase revealed the distribution of these transmitter-specific neurons in the rat. Morphologically, the two types of neurons appear similar, in that they are predominantly large multipolar cells, but they are characterized by different, overlapping distributions in the diagonal band. Glutamate decarboxylase-positive cells are scattered throughout the nucleus of the vertical limb of the diagonal band, while choline acetyltransferase-positive neurons are more numerous medially and are distributed in groups corresponding to the dorsal and ventral aspects of the nucleus. In the rostral parts of the nucleus of the horizontal limb of the diagonal band, the choline acetyltransferase-positive cells tend to be located medially, whereas caudally they spread dorsal to the nucleus to become continuous with other large cholinergic neurons in the ventral pallidum and sublenticular substantia innominata. The large majority of glutamate decarboxylase-positive neurons remain in a more ventral and lateral position within the nucleus of the horizontal limb and are particularly numerous just lateral to the diagonal band fibres as they join the medial forebrain bundle. Cholinergic neurons were estimated to be about two times more numerous than GABAergic neurons. Approximately 1% of the choline acetyltransferase-positive neurons were also glutamate decarboxylase-positive in double immunofluorescence studies, but not in sequentially stained or serial sections.