Sulpiride-induced increases in serum prolactin levels in female rats exposed prenatally to alcohol.
Sulpiride-induced increases in serum prolactin levels in female rats exposed prenatally to alcohol.
复制标题
舒必利导致产前暴露于酒精的雌性大鼠血清催乳素水平升高。
DOI：
10.1016/s0741-8329(97)00053-0
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Subramanian,MG
中科院分区：
文献类型：
--
作者：
Hannigan,JH;Hackett,JA;Tilak,J;Subramanian,MG
我们研究了产前酒精暴露对血清催乳素水平和D2多巴胺拮抗剂舒必利刺激Long-Evans大鼠催乳素释放能力的影响。从妊娠第8天（GD 8）至GD 20，对妊娠大鼠进行酒精（0、3或5 g/kg/天）插管。筛选成年雌性后代的发情周期阶段。在间情期，用舒必利（0、10、40 μg/kg）单次攻击大鼠，20 min后取躯干血。在产前暴露于任何剂量的酒精后，催乳素的平均基础血清水平比0 g/kg组低约65%，与未处理的对照组相比，35-40%的平均差异不显著。在所有产前治疗组中，舒必利均使血清催乳素水平显著增加，且呈剂量依赖性。在所有剂量的舒必利中，给予较高剂量产前酒精（5 g/kg/天）的组血清催乳素水平显著低于所有其他组。产前治疗和舒必利剂量之间没有显著的相互作用。在这项研究中，产前酒精暴露和舒必利注射对血清皮质酮水平都没有显着影响。虽然目前的结果还不清楚产前酒精暴露后催乳素水平的基线下降，总体结果表明，产前酒精暴露降低催乳素水平，但没有证据表明，它通过改变雌性大鼠下丘脑多巴胺能神经元的张力。
We examined the impact of prenatal alcohol exposure on serum prolactin levels and on the ability of the D2dopamine antagonist sulpiride to stimulate prolactin release in Long-Evans rats. Pregnant rats were intubated with alcohol (0, 3, or 5 g/kg/day) from gestational day 8 (GD8) to GD20. Adult female offspring were screened for estrous cycle stage. At diestrus, the rats were challenged with a single dose of sulpiride (0, 10, or 40 μg/kg) and trunk blood was collected 20 min later. After prenatal exposure to either dose of alcohol, mean basal serum levels of prolactin were about 65% less than the 0 g/kg group, and the 35–40% mean differences from an untreated control group were not significant. Sulpiride produced dramatic dose-dependent increases in serum prolactin levels in all prenatal treatment groups. Across all doses of sulpiride, the group given the higher dose of prenatal alcohol (5 g/kg/day) had significantly lower serum prolactin levels than all other groups. There was no significant interaction between prenatal treatment and sulpiride dose. Neither prenatal alcohol exposure nor sulpiride injections had significant effects on serum corticosterone levels in this study. Although the current results are unclear regarding a baseline decrease in prolactin levels after prenatal alcohol exposure, the overall results suggest that prenatal alcohol exposure decreases prolactin levels but there is no evidence that it does so by altering dopaminergic tone in hypothalamus of female rats.