Carnosine as a histidine source: transport and hydrolysis of exogeneous carnosine by rat intestine.
Carnosine as a histidine source: transport and hydrolysis of exogeneous carnosine by rat intestine.
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肌肽作为组氨酸来源：大鼠肠道外源肌肽的运输和水解。
DOI：
10.3177/jnsv.31.607
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发表时间：
1985
影响因子：
1.6
通讯作者：
N. Mizutani
中科院分区：
文献类型：
--
作者：
Nanaya Tamaki;T. Ikeda;S. Fujimoto;N. Mizutani
研究了 L-肌肽（β-丙氨酰-L-组氨酸）在大鼠小肠中的转运和代谢。在大鼠血清以及小肠和肝脏中发现口服肌肽，随后组氨酸增加。肌肽经口输注后10分钟，肝门静脉肌肽含量随剂量增加而增加。另一方面，组氨酸含量增加两倍，但不随剂量变化。这些结果表明，部分口服肌肽在小肠中水解为β-丙氨酸和组氨酸。肌肽酶活性存在于许多大鼠组织中，并且在存在 Mn2+ 的情况下在肾脏中最活跃。然而，在没有 Mn2+ 肌肽酶的情况下，小肠中的肌肽酶活性与肾脏中的活性水平相同。对成年大鼠小肠中肌肽酶的分布进行了研究。二肽酶沿小肠全长分布，在空肠中水解活性最大，并且位于肠粘膜的细胞质中。从肾脏纯化的抗肌肽酶制备的抗血清抑制小肠和肾脏的活性。
Transport and metabolism of L-carnosine (beta-alanyl-L-histidine) were studied in rat small intestine. Carnosine administered orally was found in rat serum as well as small intestine and liver, followed by an increase of histidine. At ten minutes after carnosine infusion per os, the carnosine content of the hepatic portal vein increased with the dose. On the other hand, the histidine content increased two-fold but did not vary with the dose. These results suggest that part of the carnosine administered orally is hydrolyzed to beta-alanine and histidine in the small intestine. Carnosinase activity was present in many rat tissues and was most active in kidney in the presence of Mn2+. However, in the absence of Mn2+ carnosinase activity in small intestine was found to be the same level as that of kidney. A study has been made of the distribution of carnosinase along the small intestine of adult rat. The dipeptidase was distributed along the whole length of the small intestine with maximum hydrolytic activity in the jejunum, and was localized in the cytosol of the intestinal mucosa. Antiserum prepared against carnosinase purified from kidney inhibited the activity of small intestine as well as that of kidney.
DOI：
10.1016/s0021-9258(17)43843-9
发表时间：
1983-12
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
V. Ganapathy;Frederick H. LeibachS
通讯作者：
V. Ganapathy;Frederick H. LeibachS