Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia
Isolation and Molecular Characterization of Leptospira interrogans and Leptospira borgpetersenii Isolates from the Urban Rat Populations of Kuala Lumpur, Malaysia
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DOI：
10.4269/ajtmh.12-0662
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发表时间：
2013-04-01
影响因子：
3.3
通讯作者：
Thong, Kwai Lin
中科院分区：
文献类型：
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作者：
Benacer, Douadi;Zain, Siti Nursheena Mohd;Thong, Kwai Lin
大鼠被认为是钩端螺旋体的主要维持宿主。本研究的目的是分离和鉴定在吉隆坡城市鼠群中传播的钩端螺旋体血清型。城市鼠300只，家鼠73%，褐家鼠27%。来自三个不同地点的30只（褐家鼠）被困。使用暗视野显微镜，20个培养物为钩端螺旋体阳性。R.家鼠为主要携带者（70%）。聚合酶链反应（PCR）证实所有分离株均为致病性钩端螺旋体。用显微镜凝集试验（MAT）鉴定了两个钩端螺旋体血清群，爪哇型和巴达维亚型。脉冲场凝胶电泳（PFGE）在城市鼠群中鉴定出两种血清型：L。爪哇型（borgpetersenii serovar Javanica）占85%;巴达维亚型问号线虫（15%）。我们的结论是，这两个血清型的主要血清型之间的城市老鼠种群在吉隆坡。尽管报告的感染率较低，但这些血清型的高致病性引起了对啮齿动物传播钩端螺旋体病所造成的公共卫生风险的关注。
Rats are considered the principal maintenance hosts of Leptospira. The objectives of this study were isolation and identification of Leptospira serovars circulating among urban rat populations in Kuala Lumpur. Three hundred urban rats (73% Rattus rattus and 27% R. norvegicus) from three different sites were trapped. Twenty cultures were positive for Leptospira using dark-field microscopy. R. rattus was the dominant carrier (70%). Polymerase chain reaction (PCR) confirmed that all isolates were pathogenic Leptospira species. Two Leptospira serogroups, Javanica and Bataviae, were identified using microscopic agglutination test (MAT). Pulsed-field gel electrophoresis (PFGE) identified two serovars in the urban rat populations: L. borgpetersenii serovar Javanica (85%) and L. interrogans serovar Bataviae (15%). We conclude that these two serovars are the major serovars circulating among the urban rat populations in Kuala Lumpur. Despite the low infection rate reported, the high pathogenicity of these serovars raises concern of public health risks caused by rodent transmission of leptospirosis.