Comparison of 10 nm filaments from three bovine tissues.
Comparison of 10 nm filaments from three bovine tissues.
复制标题
来自三种牛组织的 10 nm 细丝的比较。
DOI：
10.1016/0014-4827(80)90075-0
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发表时间：
1980
影响因子：
3.7
通讯作者：
Anderson,RA
中科院分区：
文献类型：
--
作者：
Gipson,IK;Anderson,RA
从三个牛组织中分离出10 nm细丝的富集组分，并用形态、生化和免疫学技术进行比较。我们研究了来自蹄上皮的角蛋白细丝、来自角膜上皮的10 nm细丝和来自脑白质的10 nm细丝。比较的参数和结果如下。在低离子强度(0.005 M)的缓冲液中透析8M尿素提取液后，角膜上皮细丝和角蛋白细丝重新聚合。然而，如果尿素可溶部分在含有0.17M氯化钠的相同缓冲液中透析，则可获得更高的再聚合角膜上皮细丝产量。通过细胞分离获得的脑丝在相似的处理下不会重新聚合。在十二烷基硫酸钠(十二烷基硫酸钠)聚丙烯酰胺凝胶中，三种来源的富含丝状物的蛋白质在凝胶中的图谱不同，但有一条共迁移带。通过对洗脱的共迁移蛋白进行有限的蛋白分解得到的多肽图谱显示很少的相似之处。对共同迁移蛋白的氨基酸分析显示出许多差异。抗共游走的角膜上皮细丝和脑细丝蛋白的抗体仅与自身抗原和全丝型抗体反应，而抗总角蛋白细丝蛋白的抗体仅与角蛋白细丝交叉反应。
Enriched fractions of 10 nm filaments were isolated from three bovine tissues and were compared using morphological biochemical, and immunological techniques. We studied keratin filaments from hoof epidermis, 10 nm filaments from corneal epithelium, and 10 nm filaments from brain white matter. The parameters of comparison and results were as follows.1.1. Corneal epithelial filaments and keratin filaments repolymerized after a buffered 8 M urea extract of the tissue was dialyzed against a low ionic strength (0.005 M) buffer. However, a greater yield of repolymerized corneal epithelial filaments was obtained if the urea-soluble fraction was dialyzed against the same buffer containing 0.17 M NaCl. Brain filaments harvested by cell fractionation did not repolymerize when similarly treated.2.2. Electrophoretic patterns of proteins of filament-enriched fractions from the three sources were different in sodium dodecyl sulphate (SDS) polyacrylamide gels, except for one co-migrating band.3.3. Peptide mapping by limited proteolysis of the eluted co-migrating proteins showed few similarities.4.4. Amino acid analysis of the co-migrating proteins revealed numerous differences.5.5. Antibodies to the co-migrating corneal epithelial filament and brain filament proteins reacted only with their own antigen and whole filament type, and antibody to total keratin filament protein cross-reacted only with keratin filaments.