Effect of aspirin treatment on the hypotensive effect of clonidine in rats.
Effect of aspirin treatment on the hypotensive effect of clonidine in rats.
复制标题
阿司匹林治疗对可乐定大鼠降血压作用的影响。
DOI：
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发表时间：
1983
影响因子：
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通讯作者：
P. Moore
中科院分区：
文献类型：
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作者：
B. Twohig;R. Griffiths;N. D. Barber;P. Moore
用阿司匹林（5 mg/kg i.p.5天）或盐水预处理大鼠，用戊巴比妥麻醉，并从颈动脉记录血压。通过插入股静脉的插管静脉内注射可乐定（30微克/kg）。阿司匹林预处理可显著降低可乐定的消肿作用。可乐定给药后60分钟，从动物中取出脾、心脑、肾和肺。通过在Tris缓冲液（pH 7.4）或在1 M甲酸：乙醇（1：V/v）中培养组织（1：4 w/v）来测定来自内源性底物的前列腺素生物合成。前列腺素提取到乙酸乙酯和生物测定的大鼠胃条。可乐定给药显著增加心、脑和肾中前列腺素的形成。用阿司匹林预处理的动物显示，可乐定诱导的心脏、大脑和脾脏前列腺素合成增加减少。结果提示，可乐定对麻醉大鼠的消肿作用可能部分是通过刺激中枢前列腺素的生物合成实现的。
Rats were pretreated with aspirin (5 mg/kg i.p. 5 days) or saline, anaesthetized with pentobarbitone, and blood pressure recorded from the carotid artery. Clonidine (30 micrograms/kg) was injected intravenously via a cannula inserted into the femoral vein. Aspirin pretreatment significantly reduced the hypotensive effect of clonidine. Spleen, heart brain, kidneys and lung were removed from the animals 60 min after clonidine administration. Prostaglandin biosynthesis from endogenous substrate was determined by homogenizing tissues (1:4 w/v) in either Tris buffer (pH 7.4) or in 1 M formic acid: ethanol (1:V/v). Prostaglandins were extracted into ethyl acetate and bioassayed on the rat stomach strip. Clonidine administration significantly increased the prostaglandin formation in heart, brain and kidney. Animals pretreated with aspirin showed a reduction in the clonidine-induced increase in prostaglandin synthesis in heart, brain and spleen. The results suggest that the hypotensive effect of clonidine in anaesthetized rats may in part be secondary to stimulation of central prostaglandin biosynthesis.