Infection of epithelial cells with Salmonella enterica.
Infection of epithelial cells with Salmonella enterica.
复制标题
肠沙门氏菌感染上皮细胞。
DOI：
10.1007/978-1-60327-032-8_16
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Steele-Mortimer,Olivia
中科院分区：
文献类型：
--
作者：
Steele-Mortimer,Olivia
肠炎沙门氏菌可引起多种疾病，从自限性肠胃炎到严重的全身感染。这些兼性细胞内病原体的毒力取决于它们在非吞噬细胞内侵入和复制的能力，培养的上皮细胞系统已被广泛用于剖析所涉及的分子机制。为了在体外有效入侵，细菌细胞的生长条件至关重要，因为与入侵相关的III型分泌系统（T3SS1）必须得到表达和功能。沙门氏菌入侵和复制上皮细胞的能力可以很容易地评估使用庆大霉素保护试验或免疫荧光显微镜。这里，详细介绍了我们实验室使用的协议。
Salmonella entericaserovars cause a variety of diseases ranging from self-limiting gastroenteritis to severe systemic infections. Virulence of these facultative intracellular pathogens is dependent on their ability to invade and replicate within non-phagocytic cells, and cultured epithelial cell systems have been used extensively to dissect the molecular mechanisms involved. For efficient invasionin vitro, the bacterial cell growth conditions as critical since the invasion associated type III secretion system (T3SS1) must be expressed and functional. The ability ofSalmonellato invade, and replicate within, epithelial cells can be easily assessed using a gentamicin protection assay or immunofluorescence microscopy. Here, the protocols used in our laboratory are described in detail.