Studies of Myc super-competition and clonal growth in Drosophila males and females.
Studies of Myc super-competition and clonal growth in Drosophila males and females.
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DOI：
10.17912/micropub.biology.000502
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发表时间：
2021
影响因子：
--
通讯作者：
Johnston LA
中科院分区：
文献类型：
--
作者：
Svoysky AJ;Bellah JL;Johnston LA
细胞竞争是一种细胞选择过程，由于不同适应性的细胞之间的相互作用，在生长的组织中出现。这种行为也在Myc超级竞争中观察到，其中果蝇生长中的翼盘中的健康野生型细胞被表达更高水平的Myc癌基因的附近细胞竞争。大多数关于Myc超级竞争的工作都在雄性和雌性幼虫的混合种群中进行了研究。然而，由于果蝇雄性和雌性之间的生理和遗传差异可能会影响细胞的竞争行为，我们研究了性别差异是否会影响这一过程。在这里，我们表明，男性和女性翅盘细胞受到Myc超级竞争。女性椎间盘细胞似乎比男性细胞对竞争性消除更敏感，这可能是由于性别之间基线细胞Myc水平的差异。我们还报告两性异形的细胞大小和数量之间的男性和女性生长的翼盘，是独立的竞争，翼盘和翅袋从女性比男性大，由于更大的细胞大小和细胞数量。我们建议，在细胞竞争试验中分别检查男性和女性组织可以增强我们对性别特异性途径对细胞和超级竞争的影响的理解。
Cell competition is a cell selection process that arises in growing tissues as a result of interactions between cells of different fitness. This behavior is also observed in Myc super-competition, where healthy wild type cells in growing wing discs of Drosophila are outcompeted by nearby cells that express higher levels of the Myc oncogene. Most work on Myc super-competition has examined it in mixed populations of male and female larvae. However, as physiological and genetic differences between Drosophila males and females could affect the competitive behavior of cells, we have investigated whether sex differences affect the process. Here we show that both male and female wing disc cells are subject to Myc super-competition. Female disc cells appear to be more sensitive to competitive elimination than male cells, potentially due to differences in baseline cellular Myc levels between the sexes. We also report sexual dimorphism of cell size and number between male and female growing wing discs that is independent of competition; wing discs and wing pouches from females are larger than males’ due to larger cell size and cell number. We suggest that separately examining male and female tissues in cell competition assays could enhance our understanding of the effects of sex-specific pathways on cell and super-competition.