A computer-assisted method for determining the nearest integer ratios of amino acid residues in purified proteins.
A computer-assisted method for determining the nearest integer ratios of amino acid residues in purified proteins.
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一种计算机辅助方法，用于确定纯化蛋白质中氨基酸残基的最接近整数比。
DOI：
10.1111/j.1399-3011.1974.tb02369.x
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
P. Harrison
中科院分区：
文献类型：
--
作者：
T. G. Hoy;W. Ferdinand;P. Harrison
描述了寻找纯蛋白质中最佳的完整氨基酸残基数的常规方法。该方法首先对蛋白质分子量进行最佳物理估计，并从大量氨基酸分析中得出氨基酸组成。对于给定的分子量，计算每个氨基酸的残基数±其标准误差。使用简单的统计筛选程序来消除不可靠的氨基酸。其余的用于从以前的方法开发的最小二乘分析，以找到最适合整数残基数的分子量值。还计算了拟合可靠性的估计值。借助台式计算机，找到最佳整数集的工作时间可以减少到大约 30 分钟。此外，还提供了每个整数的置信限估计。参考牛胰核糖核酸酶、马脾脱铁铁蛋白和大肠杆菌柠檬酸合酶的实验结果来说明该方法的使用。
A routine method for finding the best set of integral amino acid residue numbers in a pure protein is described. The method starts with the best physical estimate of the molecular weight of the protein, and the amino acid composition derived from a number of amino acid analyses. The number of residues of each amino acid ± its standard error is calculated for the given molecular weight. Simple statistical screening procedures are used to eliminate unreliable amino acids. The remaining ones are used in a least-squares analysis developed from previous methods, to find the value of the molecular weight that gives the best fit to integral residue numbers. An estimate of the reliability of fit is also calculated. With the aid of a desktop computer the labour of finding the the best set of integers can be reduced to about 30 minutes' work. In addition an estimate of the confidence limits for each integer is provided. The use of the method is illustrated by reference to experimental results for bovine pancreatic ribonuclease, horse spleen apoferritin and E. coli citrate synthase.