Design of fluorescent nanocapsules as ratiometric nanothermometers.
Design of fluorescent nanocapsules as ratiometric nanothermometers.
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DOI：
10.1002/chem.201402828
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发表时间：
2014-08-11
期刊：
影响因子：
--
通讯作者：
Berezin MY
中科院分区：
文献类型：
--
作者：
Zhegalova NG;Dergunov SA;Wang ST;Pinkhassik E;Berezin MY
We have developed a novel design of optical nanothermometers that can measure the surrounding temperature in the range of 20–85°C. The nanothermometers comprise of two organic fluorophores encapsulated in a crosslinked polymethacrylate nanoshell. The role of the nanocapsule shell around the fluorophores is to form a well-defined and stable microenvironment to prevent other factors besides temperature from affecting the dyes’ fluorescence. The two fluorophores feature different temperature-dependent emission profiles where a fluorophore with relatively insensitive fluorescence (rhodamine 640) serves as a reference while a sensitive fluorophore (indocyanine green) serves as a sensor. The sensitivity of the nanothermometers depends on the type of nanocapsules-forming lipid and is affected by the phase transition temperature. Both the fluorescence intensity and the fluorescence lifetime can be utilized to measure the temperature.
