A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
A LbL-Assembled Bioactive Coating Modified Nanofibrous Membrane for Rapid Tendon-Bone Healing in ACL Reconstruction
复制标题
LbL 组装的生物活性涂层改性纳米纤维膜，用于 ACL 重建中的快速腱骨愈合
DOI：
10.2147/ijn.s214359
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发表时间：
2019-01-01
影响因子：
8
通讯作者：
Zhao, Peng
中科院分区：
文献类型：
--
作者：
Han, Fei;Zhang, Peng;Zhao, Peng
简介：在前交叉韧带 (ACL) 重建中，自体腘绳肌腱移植物是一种广泛接受的替代 ACL 移植物的手术选择。但自体移植物的主要缺点是与宿主骨隧道的愈合效率低，导致手术失败。方法：本工作制备了一种用于肌腱-骨整合的仿生纳米纤维膜，该膜由聚己内酯（PCL）静电纺丝膜和壳聚糖/透明质酸（CS/HA）多层膜组成。结果：通过使用层层（LbL）自组装技术，将功能性CS/HA多层膜沉积在肌腱-骨融合体上。 PCL 纳米纤维表面能够在肌腱-骨界面局部递送基质细胞衍生因子 1 α (SDF-1 α) 和骨形态发生蛋白 2 (BMP-2)。该膜可以促进细胞增殖和募集，并诱导骨髓间充质干细胞的成骨分化和募集。结论：进一步的体内研究表明，在兔 ACL 重建模型中，使用该膜包裹自体肌腱可以提供更好的肌腱-骨整合并抑制疤痕组织形成。更重要的是，腱-骨界面的生物力学性能得到了改善。这项研究表明，这种仿生纳米纤维膜可有效改善 ACL 重建手术后的腱骨愈合。
Introduction: In anterior cruciate ligament (ACL) reconstruction, hamstring tendon autograft is a well-accepted surgical choice as an alternative ACL graft. But the main disadvantage of autograft is its inefficient healing with host bone-tunnel which will leading to surgery failure.Methods: A biomimetic nanofibrous membrane for tendon-bone integration is fabricated in this work, which is composed of polycaprolactone (PCL) electrospinning membrane and chitosan/hyaluronic acid (CS/HA) multilayers film.Results: By using layer-by-layer (LbL) self-assembly this functional CS/HA multilayer films are deposited on the surface of PCL nanofiber to enable the local delivery of stromal cell-derived factor-1 alpha (SDF-1 alpha) and bone morphogenetic protein-2 (BMP-2) in tendon-bone interface. This membrane can promote cell proliferation and recruitment, as well as inducing the osteogenic differentiation and recruitment of BMSCs.Conclusion: Further in vivo studies demonstrate that to wrap the tendon autograft using the membrane may afford superior tendon-bone integration and inhibit scar tissue formation in a rabbit ACL reconstruction model. More importantly, the biomechanical properties of the tendon-bone interface have been improved. This study shows that this biomimetic nanofibrous membrane is effective for improving tendon-bone healing after ACL reconstruction surgery.