Low concentration fluoride stimulates cell motility of epithelial cells in vitro
Low concentration fluoride stimulates cell motility of epithelial cells in vitro
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DOI：
10.2220/biomedres.30.271
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发表时间：
2009-10-01
影响因子：
1.2
通讯作者：
Arakawa, Hirohisa
中科院分区：
文献类型：
--
作者：
Arakawa, Yuki;Bhawal, Ujjal K.;Arakawa, Hirohisa
口腔粘膜组织在局部应用后可以作为长期的氟化物储存库，并将少量的氟化物保留在口腔环境中，以预防龋齿。本研究旨在探讨低浓度氟化钠（NaF）对体外培养的上皮细胞增殖和迁移的影响。使用人原代牙龈上皮细胞和人表皮HaCaT角质形成细胞。培养的上皮细胞，用从5 μ M到500 μ M的各种浓度的NaF处理，通过3-（4，5-二甲基噻唑-2-基）-5-（3-羧基甲氧基苯基）-2-（4-磺基苯基）-2H-四唑、内盐（MTS）测定、伤口愈合测定、侵袭测定和定量实时PCR来研究。MTS试验表明，在5 μ M或更高的浓度下，加入到人牙龈上皮细胞中的氟化物会提高细胞增殖。伤口愈合测定和侵袭测定证实了这一观察结果。实时荧光定量PCR显示，低浓度的氟化钠上调氟处理的细胞与对照组相比，纤维连接蛋白mRNA的表达。这些结果表明，低浓度的NaF能够诱导上皮细胞中的细胞增殖、迁移和基质产生。我们的研究结果提供了新的信息上皮细胞粘附，从而有助于牙周生理学的理解。
Oral mucosal tissue can serve as a long-term fluoride reservoir following topical application and retain a small amount of fluoride in oral environment for prevention of dental caries. The aim of this study was to determine the effect of low level sodium fluoride (NaF) on the proliferation and migration of epithelial cells in vitro. Human primary gingival epithelial cells and human epidermal HaCaT keratinocytes were used. Cultured epithelial cells, treated with various concentrations of NaF ranging from 5 mu M to 500 mu M, were investigated by 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) assay, wound healing assay, invasion assay and quantitative real-time PCR. MTS assay revealed that fluoride added to human gingival epithelial cells elevated cell proliferation at a concentration of 5 mu M or more. The wound healing assay and invasion assay confirmed this observation. Quantitative real-time PCR revealed that low concentration of NaF up-regulated fibronectin mRNA expression in fluoride-treated cells compared with controls. These results suggest that a low concentration of NaF is able to induce cell proliferation, migration, and matrix production in epithelial cells. Our results provide new information on epithelial cell adhesion and may thus aid in the understanding of periodontal physiology.