Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb
Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb
复制标题
DOI：
10.1096/fasebj.14.7.904
复制
发表时间：
2000-05-01
期刊：
影响因子：
4.8
通讯作者：
Passananti, C
中科院分区：
文献类型：
--
作者：
Fanciulli, M;Bruno, T;Passananti, C
hRPB 11是RNA聚合酶II（pol II）核心提交物，其在阿霉素（dox）处理时特异性下调。这种蛋白质的水平深刻地影响细胞分化、细胞增殖和体内致瘤性。在这里，我们描述了Che-1，一种与hRPB 11相互作用的新型人类蛋白质。Che-1具有与大肠杆菌RNA聚合酶σ-因子70和SV 40大T抗原高度同源的结构域。此外，我们报告说，车-l相互作用与视网膜母细胞瘤易感基因（Rb）的两个不同的领域。在功能上，我们表明，车-1抑制Rb的生长抑制功能，抵消Rb对E2 F1的反式激活功能的抑制作用。这些结果鉴定了一种新的结合Rb和pol II核心的蛋白质，并表明Che-1可能是转录调节复合物的一部分。
hRPB11 is a core submit of RNA polymerase II (pol II) specifically down-regulated on doxorubicin (dox) treatment. Levels of this protein profoundly affect cell differentiation, cell proliferation, and tumorigenicity in vivo. Here we describe Che-1, a novel human protein that interacts with hRPB11. Che-1 possesses a domain of high homology with Escherichia coli RNA polymerase sigma-factor 70 and SV40 large T antigen. In addition, we report that Che-l interacts with the retinoblastoma susceptibility gene (Rb) by two distinct domains. Functionally, we demonstrate that Che-1 represses the growth suppression function of Rb, counteracting the inhibitory action of Rb on the trans-activation function of E2F1. These results identify a novel protein that binds Rb and the core of pol II, and suggest that Che-l may be part of transcription regulatory complex.