The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs
The surveillance complex interacts with the translation release factors to enhance termination and degrade aberrant mRNAs
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DOI：
10.1101/gad.12.11.1665
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发表时间：
1998-06-01
影响因子：
10.5
通讯作者：
Peltz, SW
中科院分区：
文献类型：
--
作者：
Czaplinski, K;Ruiz-Echevarria, MJ;Peltz, SW
无义介导的mRNA衰变途径是进化上保守的监视途径的一个例子，该途径使细胞摆脱含有无义突变的转录物。UPF1基因的产物是公认的监视复合物的必要组成部分，该复合物识别并降解异常mRNA。最近的研究结果表明，Upf1p也增强翻译终止在一个无义密码子。本文提供的结果表明，酵母和人类形式的Upf 1 p与真核翻译终止因子eRF 1和eRF 3相互作用。与Upf1p与eRFs相互作用一致，Upf1p存在于酵母[PSI+]菌株中观察到的含有eRF1和eRF3的朊病毒样聚集体中。这些结果表明，Upf1p与肽基释放因子的相互作用可能是组装推定的监视复合物的关键事件，该监视复合物增强翻译终止，监测终止是否过早发生，并促进异常转录物的降解。
The nonsense-mediated mRNA decay pathway is an example of an evolutionarily conserved surveillance pathway that rids the cell of transcripts that contain nonsense mutations. The product of the UPF1 gene is a necessary component of the putative surveillance complex that recognizes and degrades aberrant mRNAs. Recent results indicate that the Upf1p also enhances translation termination at a nonsense codon. The results presented here demonstrate that the yeast and human forms of the Upf1p interact with both eukaryotic translation termination factors eRF1 and eRF3. Consistent with Upf1p interacting with the eRFs, the Upf1p is found in the prion-like aggregates that contain eRF1 and eRF3 observed in yeast [PSI+] strains. These results suggest that interaction of the Upf1p with the peptidyl release factors may be a key event in the assembly of the putative surveillance complex that enhances translation termination, monitors whether termination has occurred prematurely, and promotes degradation of aberrant transcripts.