Cytotoxic and genotoxic effects of the azo-dye p-dimethylaminoazobenzene in mice:: A time-course study
Cytotoxic and genotoxic effects of the azo-dye p-dimethylaminoazobenzene in mice:: A time-course study
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DOI：
10.1016/j.mrgentox.2005.06.011
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发表时间：
2005-11-10
影响因子：
1.9
通讯作者：
Khuda-Bukhsh, AR
中科院分区：
文献类型：
--
作者：
Biswas, SJ;Khuda-Bukhsh, AR
在小鼠中评估了在7、15、30、60、90和120天期间长期喂食偶氮染料对二甲氨基偶氮苯（p-DAB）的细胞毒性和遗传毒性效应。用于遗传毒性分析的终点是骨髓细胞中的染色体畸变（CA）、微核（MN）和有丝分裂指数（MI）以及雄性性腺中的精子头异常（SHA）。定期检测毒性标志酶如谷草转氨酶（GOT）、谷丙转氨酶（GPT）、酸性磷酸酶（ACP）和碱性磷酸酶（ALKP）的活性以及脂质过氧化（LPO）。与正常的未处理对照组相比，慢性喂养p-DAB产生染色体畸变、核异常和精子头异常的数量增加，通常以时间依赖性的方式持续到60天，之后异常持续存在，但相当不稳定。然而，尽管在相应的p-DAB喂养的小鼠中酶活性也有一些明显的调节，但这些调节并不严格依赖于时间。(c)2005 Elsevier B. V.保留所有权利。
The cytotoxic and genotoxic effects of chronic feeding of the azo-dye p-dimethylaminoazobenzene (p-DAB) during 7, 15, 30, 60, 90 and 120 days have been assessed in mice. The endpoints used for genotoxic analysis were chromosome aberrations (CA), micronuclei (MN) and mitotic index (MI) in bone-marrow cells, and sperm-head abnormality (SHA) in male gonads. The activities of marker enzymes for toxicity, such as glutamate oxalo-acetate transaminase (GOT), glutamate pyruvate transaminase (GPT), acid phosphatase (ACP) and alkaline phosphatase (ALKP) were also assayed periodically, as was lipid peroxidation (LPO). Chronic feeding of p-DAB produced increased numbers of chromosome aberrations, nuclear anomalies and sperm-head abnormalities, as compared with normal untreated controls, generally in a time-dependent manner until 60 days, after which the anomalies persisted, but rather erratically. However, although there was some noticeable modulation in enzyme activities in the corresponding p-DAB-fed mice as well, these were not strictly time-dependent. (c) 2005 Elsevier B.V. All rights reserved.