DESIGN AND FABRICATION OF BIODEGRADABLE POLYMER DEVICES TO ENGINEER TUBULAR TISSUES
DESIGN AND FABRICATION OF BIODEGRADABLE POLYMER DEVICES TO ENGINEER TUBULAR TISSUES
复制标题
DOI：
10.1177/096368979400300209
复制
发表时间：
1994-03-01
影响因子：
3.3
通讯作者：
LANGER, R
中科院分区：
文献类型：
--
作者：
MOONEY, DJ;ORGAN, G;LANGER, R
通过在聚合物输送装置上移植细胞来设计新组织是缓解供体组织巨大短缺的一种方法。然而，有必要制造细胞递送装置，将细胞递送到给定的位置，并促进从移植细胞和宿主组织形成特定的组织结构。本报告描述了一种用于引导管状血管组织发育的聚合物装置的设计和制造，该装置可用于各种组织的工程，包括肠、血管、气管和输尿管。在体内外，形成了聚(D，L-乳酸-羟基乙酸共聚)多孔膜，并制成了具有抗压力的管状结构。这些装置在植入受体动物体内后促进纤维血管组织的生长，从而形成血管化的管状组织。为了研究这些装置作为细胞输送装置的用途，在体外将肠上皮细胞接种到这些装置上。肠细胞被发现附着在这些装置上，并形成一个有组织的上皮细胞层。这些结果表明，这些装置可能是移植细胞和设计新的肾小管组织的合适载体。
Engineering new tissues by transplanting cells on polymeric delivery devices is one approach to alleviate the vast shortage of donor tissue. However, it will be necessary to fabricate cell delivery devices that deliver cells to a given location and promote the formation of specific tissue structures from the transplanted cells and the host tissue. This report describes the design and fabrication of a polymeric device for guiding the development of tubular vascularized tissues, which may be useful for engineering a variety of tissues including intestine, blood vessels, tracheas, and ureters. Porous films of poly (D,L-lactic-co-glycolic acid) have been formed and fabricated into tubes capable of resisting compressional forces in vitro and in vivo. These devices promote the ingrowth of fibrovascular tissue following implantation into recipient animals, resulting in a vascularized, tubular tissue. To investigate the utility of these devices as cell delivery devices, enterocytes (intestinal epithelial cells) were seeded onto the devices in vitro. Enterocytes were found to attach to these devices and-form an organized epithelial cell layer. These results suggest that these devices may be an appropriate delivery vehicle for transplanting cells and engineering new tubular tissues.