Intranasal vaccination with ISCOMATRIX® adjuvanted influenza vaccine
Intranasal vaccination with ISCOMATRIX® adjuvanted influenza vaccine
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DOI：
10.1016/s0264-410x(02)00545-5
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发表时间：
2003-02-14
期刊：
影响因子：
5.5
通讯作者：
Pearse, M
中科院分区：
文献类型：
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作者：
Coulter, A;Harris, R;Pearse, M
通过粘膜递送能够引发针对呼吸道疾病的保护性免疫反应的灭活疫苗一直是疫苗学家的长期目标。与目前通过肠胃外途径递送的疫苗相比，此类疫苗将成为针对呼吸道疾病的更合适的疫苗接种方式。鼻内递送灭活流感疫苗加上 ICOMATRIX(R) (INIX) 佐剂，简单地混合在一起，能够在小鼠中诱导血清血凝抑制 (HAI) 滴度，远远优于皮下递送的无佐剂疫苗所获得的滴度。此外，INIX 佐剂疫苗可在肺、鼻道和大肠中通过鼻内诱导粘膜 IgA 反应，并产生高水平的血清 IgA。在绵羊中鼻内递送 IMX 佐剂流感疫苗在血清和鼻分泌物中产生的抗体反应超过了皮下注射未佐剂疫苗所获得的水平。这些观察结果表明，通过鼻内接种 IMX 佐剂灭活疫苗可能会诱导人类对流感的有效免疫力。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
Mucosal delivery of inactivated vaccines that are able to elicit protective immune responses against respiratory diseases has been a long time goal of vaccinologists. Such vaccines would enable a more appropriate means of vaccination against respiratory diseases than those currently delivered by a parenteral route. The intranasal delivery of inactivated influenza vaccine plus the ISCOMATRIX(R) (INIX) adjuvant, simply mixed together, was able to induce serum haemagglutination inhibition (HAI) titres in mice far superior to those obtained with unadjuvanted vaccine delivered subcutaneously. Furthermore, the INIX adjuvanted vaccine delivered intranasally induced mucosal IgA responses in the lung, nasal passages and large intestine, together with high levels of serum IgA. Intranasal delivery of IMX adjuvanted influenza vaccine in sheep gave antibody responses in both serum and nasal secretions that surpassed the levels obtained with unadjuvanted vaccine administered subcutaneously. These observations suggest that it may be possible to induce effective immunity to influenza in humans by intranasal vaccination with an IMX adjuvanted inactivated vaccine. (C) 2002 Elsevier Science Ltd. All rights reserved.