Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014
Characterization of a moderately pathogenic pseudorabies virus variant isolated in China, 2014
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2014 年中国分离的中度致病性伪狂犬病病毒变种的特征
DOI：
10.1016/j.meegid.2018.12.016
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发表时间：
2019-03-01
影响因子：
3.2
通讯作者：
Du, Yijun
中科院分区：
文献类型：
--
作者：
Zhou, Mingming;Wu, Xiangju;Du, Yijun
在这项研究中，我们报告了2014年从中国山东省潍坊市一个接种了bartha - k61疫苗的猪场分离到的一株中等致病性伪狂犬病毒（PRV）变体。猪场患病仔猪表现为厌食、体重减轻和神经系统症状，但未死亡。基因序列比对表明，该基因属于一个新的PRV突变株，与其他PRV菌株相比，约有15%的氨基酸位点发生突变、缺失和插入。与Bartha-K61疫苗株相比，gD基因有2个氨基酸插入和10个氨基酸突变。TK和gM基因与一株高致病性PRV TJ相同。病毒分离、实验室攻毒、血清学检测和组织病理学检查证实，该疾病的病原为PRV SD1404，是一种中等致病性毒株，可导致仔猪生病但不死亡。PRV SD1404株与其他报道的PRV不同，应引起重视，以免造成经济损失。
In this study, we reported a moderately pathogenic pseudorabies virus (PRV) variant isolated from one Bartha-K61-vaccinated pig farm in Weifang, Shandong Province, China, 2014. The sick piglets in the farm were characterized by anorexia, weight loss and neurologic symptoms but did not die. Sequence alignment of the gE gene indicated that it belonged to a new mutated PRV strain and about 15% amino acid sites had mutations, deficiencies and insertions compared to the other PRV strains. The gD gene had two amino acid insertions and ten amino acid mutations in comparison with the Bartha-K61 vaccine strain. The TK and gM genes were the same as one highly pathogenic PRV TJ strain. Evidence from virus isolation, laboratory challenge, serological detection and histopathologic examination confirmed that the etiological agent of the disease is PRV SD1404, which is a moderately pathogenic strain and causes piglets to be sick but not to die. PRV SD1404 strain is different from other reports and should be paid more attention to avoid economic losses.