Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytes
Chronic ethanol feeding to rats decreases adiponectin secretion by subcutaneous adipocytes
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DOI：
10.1152/ajpendo.00387.2006
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发表时间：
2007-02-01
影响因子：
5.1
通讯作者：
Nagy, Laura E.
中科院分区：
文献类型：
--
作者：
Chen, Xiaocong;Sebastian, Becky M.;Nagy, Laura E.
长期给小鼠和大鼠喂食乙醇会降低血清脂联素浓度，脂联素治疗可减轻慢性乙醇引起的肝损伤。尽管很明显脂联素降低具有病理生理学重要性，但长期乙醇降低脂联素的机制尚不清楚。在这里，我们研究了长期乙醇喂养对脂肪组织脂联素表达和分泌的影响。给大鼠喂食 36% Lieber-DeCarli 乙醇的液体饮食或配对喂养对照饮食 4 周。慢性乙醇喂养降低了皮下脂肪组织中脂联素mRNA，但对脂联素蛋白没有影响。慢性乙醇喂养也减少了分离的皮下和腹膜后脂肪细胞的脂联素分泌，尽管来自乙醇和配对喂养的大鼠的皮下脂肪细胞之间维持相同的细胞内脂联素浓度。布雷菲德菌素 A 治疗抑制了配对喂养大鼠皮下脂肪细胞的脂联素分泌，但在乙醇喂养后效果甚微。在配对喂养大鼠的皮下脂肪细胞中，相对于质膜，脂联素在内质网（ER）/高尔基体中富集；然而，在长期喂食乙醇后，脂联素在质膜和内质网/高尔基体部分之间均匀分布。总之，长期摄入乙醇会损害皮下和腹膜后脂肪细胞的脂联素分泌。长期乙醇喂养后，分泌受损可能导致脂联素浓度降低。
Chronic ethanol feeding to mice and rats decreases serum adiponectin concentration and adiponectin treatment attenuates chronic ethanol-induced liver injury. Although it is clear that lowered adiponectin has pathophysiological importance, the mechanisms by which chronic ethanol decreases adiponectin are not known. Here, we have investigated the impact of chronic ethanol feeding on adiponectin expression and secretion by adipose tissue. Rats were fed a 36% Lieber-DeCarli ethanol-containing liquid diet or pair-fed control diet for 4 wk. Chronic ethanol feeding decreased adiponectin mRNA but had no effect on adiponectin protein in subcutaneous adipose tissue. Chronic ethanol feeding also reduced adiponectin secretion by isolated subcutaneous and retroperitoneal adipocytes despite the maintenance of equivalent intracellular concentrations of adiponectin between subcutaneous adipocytes from ethanol- and pair-fed rats. Treatment with brefeldin A suppressed adiponectin secretion by subcutaneous adipocytes from pair-fed rats but had little effect after ethanol feeding. In subcutaneous adipocytes from pair-fed rats, adiponectin was enriched in endoplasmic reticulum (ER)/Golgi relative to plasma membrane; however, after chronic ethanol feeding, adiponectin was equally distributed between plasma membrane and ER/Golgi fractions. In conclusion, chronic ethanol feeding impaired adiponectin secretion by subcutaneous and retroperitoneal adipocytes; impaired secretion likely contributes to decreased adiponectin concentrations after chronic ethanol feeding.