Identification of adult midgut precursors in Drosophila
Identification of adult midgut precursors in Drosophila
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DOI：
10.1016/j.gep.2010.08.005
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发表时间：
2011-01-01
影响因子：
1.2
通讯作者：
Beehler-Eyans, Ryan
中科院分区：
文献类型：
--
作者：
Micchelli, Craig A.;Sudmeier, Lisa;Beehler-Eyans, Ryan
成年果蝇的中肠被认为是由胚胎发生时指定的内胚层雏形产生的。先前的研究报道了在胚胎和幼虫中肠组织中存在被称为成体中肠前体（amp）的单个细胞以及“中肠岛”或“胰岛”。然而，祖细胞群和成体中肠细胞之间的确切关系尚未被描述。利用分子标记和定向细胞谱系追踪的结合，我们提供了证据，证明成虫中肠是由胚胎/幼虫过渡过程中存在的一个分子上不同的单细胞群体产生的。amp存在于幼虫中肠的一个明显的基础位置，在那里它们一直存在于随后的所有幼虫和蛹阶段，直到成年。至少有五个阶段的AMP活性与中肠的逐步形成过程有关。我们的数据显示，在幼虫期amp产生推定的成体上皮；在蛹期amp对最终的大小、细胞数量和形态有影响。最后，基因筛选导致蜕皮激素受体作为AMP扩增的调节剂的鉴定。(c) 2010 Elsevier B.V.保留所有权利。
The adult Drosophila midgut is thought to arise from an endodermal rudiment specified during embryogenesis. Previous studies have reported the presence of individual cells termed adult midgut precursors (AMPs) as well as "midgut islands" or "islets" in embryonic and larval midgut tissue. Yet the precise relationship between progenitor cell populations and the cells of the adult midgut has not been characterized. Using a combination of molecular markers and directed cell lineage tracing, we provide evidence that the adult midgut arises from a molecularly distinct population of single cells present by the embryonic/larval transition. AMPs reside in a distinct basal position in the larval midgut where they remain through all subsequent larval and pupal stages and into adulthood. At least five phases of AMP activity are associated with the stepwise process of midgut formation. Our data shows that during larval stages AMPs give rise to the presumptive adult epithelium; during pupal stages AMPs contribute to the final size, cell number and form. Finally, a genetic screen has led to the identification of the Ecdysone receptor as a regulator of AMP expansion. (c) 2010 Elsevier B.V. All rights reserved.