Age-related Macular Degeneration Is Associated with Increased Proportion of CD56D+ T Cells in Peripheral Blood
Age-related Macular Degeneration Is Associated with Increased Proportion of CD56D+ T Cells in Peripheral Blood
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DOI：
10.1016/j.ophtha.2013.04.014
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发表时间：
2013-11-01
期刊：
影响因子：
13.7
通讯作者：
Nissen, Mogens Holst
中科院分区：
文献类型：
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作者：
Faber, Carsten;Singh, Amardeep;Nissen, Mogens Holst
目的：研究年龄相关的t细胞腔室变化与年龄相关性黄斑变性（AMD）患病率之间的关系。设计：病例对照研究。参与者：共纳入117例AMD病例和106例对照。方法：对新鲜抽取的外周血标本进行流式细胞术分析t细胞群。检测血浆中抗巨细胞病毒（CMV）免疫球蛋白（Ig） G和补体因子H (CFH) Y402H基因型。根据临床年龄相关性黄斑病变分期系统诊断AMD。主要结局指标：衰老T细胞频率与AMD患病率之间的关系。结果：AMD的患病率与t细胞室明显的年龄相关变化有关。具体来说，AMD患者表达CD56表面标记物的CD28(-) T细胞频率增加（患者34.9% vs老年对照组25.8%；P=0.002）。在校正CFH基因型、抗巨细胞病毒IgG阳性、年龄、性别和吸烟史后，CD56(+)CD28(-) T细胞最高五分位数的参与者存在AMD的比值比（OR）为3.2（95%可信区间[CI]， 1.2-8.8）。对于至少有1个CFH H402风险等位基因且CD56(+)CD28(-) T细胞中位水平以上的人，AMD存在的校正OR从3.5 （95% CI, 1.5-8.1）增加到13.3 （95% CI, 3.3-53.6）。结论：我们发现AMD患者循环老化CD56(+)CD28(-) T细胞水平升高。虽然这支持了AMD是一种全身性疾病的观点，但它也表明适应性免疫系统参与了其发病机制。(C) 2013年由美国眼科学会发布。
Purpose: To examine the association between age-related changes in the T-cell compartment and prevalence of age-related macular degeneration (AMD).Design: Case-control study.Participants: A total of 117 AMD cases and 106 controls were included prospectively.Methods: Fresh-drawn peripheral blood samples were processed for flow cytometric analysis of T-cell populations. Plasma samples were analyzed for anti-cytomegalovirus (CMV) immunoglobulin (Ig) G and complement factor H (CFH) Y402H genotype. The diagnosis of AMD was made according to the Clinical Age-Related Maculopathy Staging System.Main Outcome Measures: Association between frequency of aged T cells and prevalence of AMD.Results: The prevalence of AMD was associated with distinct age-related changes in the T-cell compartment. Specifically, the patients with AMD had an increased frequency of CD28(-) T cells that expressed the CD56 surface marker (patients, 34.9% vs. aged controls, 25.8%; P=0.002). Participants in the highest tertile of CD56(+)CD28(-) T cells had an odds ratio (OR) for the presence of AMD of 3.2 (95% confidence interval [CI], 1.2-8.8) after adjustment for CFH genotype, anti-CMV IgG positivity, age, sex, and smoking history. The adjusted OR of the presence of AMD for persons having at least 1 CFH H402 risk allele increased from 3.5 (95% CI, 1.5-8.1) to 13.3 (95% CI, 3.3-53.6) for persons with at least 1 CFH H402 risk allele and above the median level of CD56(+)CD28(-) T cells.Conclusions: We found increased levels of circulating aged CD56(+)CD28(-) T cells in patients with AMD. Although this supports the notion of AMD as a systemic disease, it also suggests that the adaptive immune system is implicated in its pathogenesis. (C) 2013 by the American Academy of Ophthalmology.