Differential effect of kinase A and C blockers on lordosis facilitation by progesterone and its metabolites in ovariectomized estrogen-primed rats
Differential effect of kinase A and C blockers on lordosis facilitation by progesterone and its metabolites in ovariectomized estrogen-primed rats
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DOI：
10.1016/j.yhbeh.2005.08.011
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发表时间：
2006-03-01
影响因子：
3.5
通讯作者：
Beyer, C
中科院分区：
文献类型：
--
作者：
González-Flores, O;Ramírez-Orduña, JM;Beyer, C
Dose response curves for lordosis behavior was obtained for progesterone (P) and its two ring A-reduced metabolites: 5 alpha-pregnanedione (alpha-DHP) and 5 alpha,3 alpha-pregnanolone (5 alpha,3 alpha-Pgl) by infusing these progestins in the right lateral ventricle (rlv) of ovariectomized (ovx) estradiol-treated rats (2 mu g estradiol benzoate; EB), 40 h before intracerebro-ventricular (icv) injection. Effective doses 50 (ED50) revealed that ring A-reduced progestins were more potent than P itself to induce lordosis behavior. Two dose levels, one producing the maximal effect and the other one producing a submaximal response (ED50-ED60), were selected for testing the capacity of RpAMPS, a kinase A blocker, and H7, a kinase C blocker, to modify the response to the three progestins. rlv injection of RpAMPS significantly depressed the lordosis response to the two dose levels of P and alpha-DHP but failed to significantly inhibit that of 5 alpha,3 alpha-Pgl. The administration of H7 prevented the effect of both 5 alpha-reduced progestins without affecting the response to P. The results suggest that P and its ring A-reduced metabolites stimulate lordosis behavior through different cellular mechanisms: P acting mainly through the cAMP-kinase system; alpha-DHP through both kinase A and kinase C signaling pathways and 5 alpha,3 alpha-Pgl through the kinase C system. (C) 2005 Elsevier Inc. All rights reserved.
