Genetic and biological variation in equine infectious anemia virus rev correlates with variable stages of clinical disease in an experimentally infected pony
Genetic and biological variation in equine infectious anemia virus rev correlates with variable stages of clinical disease in an experimentally infected pony
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DOI：
10.1006/viro.2000.0696
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发表时间：
2001-01-05
期刊：
影响因子：
3.7
通讯作者：
Carpenter, S
中科院分区：
文献类型：
--
作者：
Belshan, M;Baccam, P;Carpenter, S
在马传染性贫血病毒（EIAV）的调节蛋白Rev的遗传和生物学变异进行了研究，在整个临床动态的疾病过程中，实验感染的小马。感染强毒EIAV（Wyo）后，小马经历了一个可变的病程，包括感染后12天（DPI）的急性发热发作，多次反复发热发作，直到135 DPI，延长的亚临床期，和两次晚期发热发作。从接种物和连续血清样品中分离病毒RNA，并扩增rev外显子2/gp 45重叠ORF，克隆并测序。在整个感染过程中发现了新的变体，遗传分析表明Rev和gp 45 ORF都处于选择压力下。接种物中占主导地位的Rev变体R1在感染后早期（直至35 DPI）仍占主导地位;然而，R1在反复发热期（67-135 DPI）被新的主导变体取代。R1重新出现的主要变异在无发热期间，但一个新的主要变异，R93，与后期发热发作。在反复发热和晚期发热期间占主导地位的Rev变体具有显著高于急性和无发热期间占主导地位的变体的Rev介导的核输出活性。Rev活性与临床疾病的不同阶段之间存在统计学相关性。总之，这些结果表明，rev的遗传和生物学变异可能是EIAV疾病进展的一个促成因素。(C)北京：科学出版社.
Genetic and biological variation in the regulatory protein Rev of equine infectious anemia virus (EIAV) were examined throughout a clinically dynamic disease course of an experimentally infected pony. Following infection with the virulent EIAV(Wyo), the pony underwent a Variable disease course, including an acute fever episode at 12 days postinfection (DPI), multiple recurrent fever episodes until 135 DPI, a prolonged subclinical period, and two late fever episodes. Viral RNA was isolated from the inoculum and sequential sera samples, and the rev exon 2/gp45 overlapping ORFs were amplified, cloned, and sequenced. Novel variants were found throughout infection, and genetic analyses indicated that both the Rev and gp45 ORFs were under selective pressure. The Rev variant predominant in the inoculum, R1, remained predominant during the early periods following infection (until 35 DPI); however, R1 was replaced by new predominant variants during the recurrent fever period (67-135 DPI). R1 reemerged as the predominant variant during the afebrile period, but a new predominant variant, R93, was associated with the late fever episodes. Rev Variants predominant during recurrent febrile and late-febrile periods had significantly higher Rev-mediated nuclear export activity than the variants predominant during the acute and afebrile periods. Statistical correlation was found between Rev activity and different stages of clinical disease. Together, these results suggest that genetic and biological variation in rev may be a contributing factor in EIAV disease progression. (C) 2001 Academic Press.