Urinary Level of Liver-Type Fatty Acid Binding Protein Reflects the Degree of Tubulointerstitial Damage in Polycystic Kidney Disease.
Urinary Level of Liver-Type Fatty Acid Binding Protein Reflects the Degree of Tubulointerstitial Damage in Polycystic Kidney Disease.
复制标题
尿肝型脂肪酸结合蛋白水平反映了多囊肾病肾小管间质损伤的程度。
DOI：
10.1159/000495389
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Kamijo-Ikemori A.
中科院分区：
文献类型：
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作者：
Watanabe S;Ichikawa D;Sugaya T;Ohata K;Inoue K;Hoshino S;Kimura K;Shibagaki Y;Kamijo-Ikemori A.
背景/目的多囊肾病(PKD)是一种常见的进展性和遗传性肾脏疾病。虽然某些成像方法对PKD的诊断和分期有用，但它们不能充分监测间质炎症和纤维化的严重程度。因此，本研究探讨了尿中肝型脂肪酸结合蛋白(L-FABP)作为PKD间质炎症和纤维化指标的价值。以年龄和性别匹配的SD大鼠(SD)(n=34)作为对照。分别于8、12、16、20、24周龄取尿标本，分别于8、16、20、24周龄取血和肾组织。结果与正常对照组相比，模型组大鼠肾小管上皮细胞增殖和间质炎症程度均呈线性增加。8~20周龄PCK大鼠肾间质纤维化呈加重趋势，20周龄达高峰。PCK大鼠尿L-FABP水平随增龄呈线性增加，12、16、20、24周显著高于对照组。肾小管间质损伤程度与肾小管间质损伤程度呈正相关，16周龄尿L-FABP水平与20周龄大鼠肾脏总体积显著相关。结论尿L-FABP可反映肾小管间质损害的进展情况，可作为监测肾小管间质病变进展的有用指标。
Background/AimsPolycystic kidney disease (PKD) is a common, progressive, and heritable type of kidney disease. Although certain imaging modalities are useful for the diagnosis and staging of PKD, they cannot adequately monitor the severity of interstitial inflammation and fibrosis. Therefore, the present study evaluated the urinary level of liver-type fatty acid binding protein (L-FABP) as a marker of interstitial inflammation and fibrosis in PKD.MethodsMale PCK/CrljCrl-Pkhd1pck/Crl (PCK) rats (n= 34) were used as an animal model of the PKD. Age-and sex-matched Sprague–Dawley rats (SD)(n= 34) were used as controls. Urine samples were obtained from the rats at 8, 12, 16, 20, and 24 weeks of age, and the sera and kidney tissues were obtained at 8, 16, 20, and 24 weeks of age.ResultsAll PCK rats developed cysts, and the degrees of tubular epithelial cell proliferation and interstitial inflammation increased linearly with age in these model rats relative to the controls. Interstitial fibrosis tended to increase in the PCK rats from 8 to 20 weeks of age, and revealed a peak level at 20 weeks. The urinary L-FABP levels increased linearly with age in the PCK rats, and the levels at 12, 16, 20, and 24 weeks were significantly higher than those in the controls. The urinary levels of L-FABP in the PCK rats correlated significantly with the severity of tubulointerstitial damage; specifically, we observed a significant correlation of the urinary levels at 16 weeks of age with the total kidney volume at 20 weeks. In contrast, both PCK and SD rats exhibited similar serum levels of L-FABP.ConclusionUrinary L-FABP reflects the progression of tubulointerstitial damage, and therefore, may be a useful marker for monitoring the progression of PKD.