Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage
Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage
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玻璃体内注射贝伐单抗可减轻早期实验性糖尿病成年大鼠的糖尿病视网膜病变
DOI：
10.1155/2018/9216791
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发表时间：
2018-05
影响因子：
4.3
通讯作者：
Zou Ying-Ying
中科院分区：
文献类型：
--
作者：
Lv Jiao;Chen Miao-Miao;Mu Zhi-Hao;Wang Fang;Qian Zhong-Yi;Zhou Lei;Guo Qiu-Ting;Zhao Zhi-Min;Pan Yu-Ping;Liao Xin-Yu;Yang Zhi-Hong;Cai Ning;Li Shu-De;Zou Ying-Ying
糖尿病视网膜病变是导致失明的主要原因，但其治疗非常有限。抗VEGF药物已广泛应用于眼部疾病的治疗，但其对糖尿病视网膜病变的影响及其机制仍有待深入研究。为了阐明抗VEGF治疗的作用，我们试图确定贝伐单抗对成年大鼠诱导糖尿病的第3周至第9周糖尿病神经血管变化的影响。视网膜神经血管的变化包括VEGF、nNOS、iNOS、eNOS和NO的表达增加。在注射贝伐珠单抗的糖尿病大鼠中，神经节细胞丢失和视网膜厚度改变得到改善。在这方面，上述生物标志物的免疫荧光标记显著降低。沿着，Western印迹证实贝伐单抗治疗与VEGF、Flk-1和cAMP反应元件结合和蛋白激酶C蛋白表达的降低相关。目前的结果表明，贝伐单抗治疗的早期阶段的视网膜病变可能会改善视网膜病变，其中VEGF/Flk-1信号已被证明在这里发挥重要作用。
Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role.
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影响因子：
1.7
作者：
H. Kimura;H. Esumi
通讯作者：
H. Kimura;H. Esumi
影响因子：
81.5
作者：
T. Wong;C. Cheung;M. Larsen;Sanjay Sharma;R. Simó
通讯作者：
T. Wong;C. Cheung;M. Larsen;Sanjay Sharma;R. Simó
影响因子：
17.8
作者：
Simo, Rafael;Hernandez, Cristina
通讯作者：
Hernandez, Cristina
影响因子：
5.3
作者：
Croll, SD;Ransohoff, RM;Wiegand, SJ
通讯作者：
Wiegand, SJ
影响因子：
2.6
作者：
Peipei Zhang;Zhanyu Zhou
通讯作者：
Peipei Zhang;Zhanyu Zhou