The Expression of BTS-2 Enhances Cell Growth and Invasiveness in Renal Cell Carcinoma
The Expression of BTS-2 Enhances Cell Growth and Invasiveness in Renal Cell Carcinoma
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DOI：
10.21873/anticanres.11637
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发表时间：
2017-06-01
影响因子：
2
通讯作者：
Yasui, Wataru
中科院分区：
文献类型：
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作者：
Quoc Thang Pham;Oue, Naohide;Yasui, Wataru
背景：肾细胞癌（RCC）是发达国家最常见的癌症之一。骨髓基质细胞抗原2（BST 2）基因编码BST 2跨膜糖蛋白，在多种癌症类型中过表达。本研究分析了BST 2在肾细胞癌中的表达和功能。材料与方法：采用免疫组化方法检测123例肾癌组织中BST 2的表达。RNA干扰用于抑制RCC细胞系中BST 2的表达。结果：免疫组化结果显示，123例肾细胞癌组织中32%的组织表达BST 2。BST 2表达与肿瘤分期呈正相关。此外，BST 2表达是RCC患者生存的独立预测因子。与阴性对照siRNA转染细胞相比，BST 2 siRNA转染的Caki-1细胞显示出显著降低的细胞生长和侵袭活性。结论：BST 2在肾癌的发生发展中起重要作用。由于BST 2在细胞膜上表达，因此BST 2是RCC的良好治疗靶点。
Background: Renal cell carcinoma (RCC) is one of the most common types of cancer in developed countries. Bone marrow stromal cell antigen 2 (BST2) gene, which encodes BST2 transmembrane glycoprotein, is overexpressed in several cancer types. In the present study, we analyzed the expression and function of BST2 in RCC. Materials and Methods: BST2 expression was analyzed by immunohistochemistry in 123 RCC cases. RNA interference was used to inhibit BST2 expression in a RCC cell line. Results: Immunohistochemical analysis showed that 32% of the 123 RCC cases were positive for BST2. BST2 expression was positively associated with tumour stage. Furthermore, BST2 expression was an independent predictor of survival in patients with RCC. BST2 siRNA-transfected Caki-1 cells displayed significantly reduced cell growth and invasive activity relative to negative control siRNA-transfected cells. Conclusion: These results suggest that BST2 plays an important role in the progression of RCC. Because BST2 is expressed on the cell membrane, BST2 is a good therapeutic target for RCC.