Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress.
Genome-wide analysis of the CaHsp20 gene family in pepper: comprehensive sequence and expression profile analysis under heat stress.
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辣椒CaHsp20基因家族的全基因组分析：热胁迫下的综合序列和表达谱分析
DOI：
10.3389/fpls.2015.00806
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发表时间：
2015
影响因子：
5.6
通讯作者：
Lu MH
中科院分区：
文献类型：
--
作者：
Guo M;Liu JH;Lu JP;Zhai YF;Wang H;Gong ZH;Wang SB;Lu MH
HSP20基因都存在于所有植物物种中，并通过防止不可逆的变性蛋白质的不可逆聚集来减轻热应激和增强植物的耐热性。然而，对于胡椒（Capsicum annuum L.）中的CAHSP20基因家族知之甚少，这是一种重要的蔬菜作物，具有温带，但热敏感。在这项研究中，还基于其分子量，然后将其基因结构，基因组重复，系统发育关系和相互作用网络鉴定出35个推定的胡椒HSP20基因（CAHSP20）。使用半定量RT-PCR测量了在热应力条件下，来自热耐剂线R9的四个不同组织（根，茎，叶和花）中CAHSP20基因的表达模式。大多数CAHSP20基因的转录本在正常温度条件下的所有四个组织中保持低水平，但受热应力高度诱导，而CAHSP16.6B，16.7和23.8的表达仅在特定的组织中检测到23.8像其他CAHSP20基因一样对热应激如此敏感。此外，与热耐剂线R9中的那些相比，热应激下热敏线B6中大多数CAHSP20基因的表达峰是滞后的，几个CAHSP20基因（CAHSP16.4，18.2a，18.7，18.7，21.2，21.2，21.2，22.0，25.0，25.8 and 25.8，and 25.8，and 25.8，and 25.8，and 25.8，和25.8，和25.8，和25.8，和25.0，25.8，和25.0，25.8，和25.0，25.8，和25.8，和25.0，25.0，25.0，25.0，25.2 a。 ）在B6和R9线中均显示出较高的表达水平。这些数据表明，CAHSP20基因可能参与胡椒中的热应力和防御反应，这为CAHSP20在辣椒获得的热果实形成时提供了进一步的功能分析。
The Hsp20 genes are present in all plant species and play important roles in alleviating heat stress and enhancing plant thermotolerance by preventing the irreversible aggregation of denaturing proteins. However, very little is known about the CaHsp20 gene family in pepper (Capsicum annuum L.), an important vegetable crop with character of temperate but thermosensitive. In this study, a total of 35 putative pepper Hsp20 genes (CaHsp20s) were identified and renamed on the basis of their molecular weight, and then their gene structure, genome location, gene duplication, phylogenetic relationship, and interaction network were also analyzed. The expression patterns of CaHsp20 genes in four different tissues (root, stem, leaf, and flower) from the thermotolerant line R9 under heat stress condition were measured using semi-quantitative RT-PCR. The transcripts of most CaHsp20 genes maintained a low level in all of the four tissues under normal temperature condition, but were highly induced by heat stress, while the expression of CaHsp16.6b, 16.7, and 23.8 were only detected in specific tissues and were not so sensitive to heat stress like other CaHsp20 genes. In addition, compared to those in thermotolerant line R9, the expression peak of most CaHsp20 genes in thermosensitive line B6 under heat stress was hysteretic, and several CaHsp20 genes (CaHsp16.4, 18.2a, 18.7, 21.2, 22.0, 25.8, and 25.9) showed higher expression levels in both line B6 and R9. These data suggest that the CaHsp20 genes may be involved in heat stress and defense responses in pepper, which provides the basis for further functional analyses of CaHsp20s in the formation of pepper acquired thermotoleance.