Pretilachlor has the potential to induce endocrine disruption, oxidative stress, apoptosis and immunotoxicity during zebrafish embryo development
Pretilachlor has the potential to induce endocrine disruption, oxidative stress, apoptosis and immunotoxicity during zebrafish embryo development
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DOI：
10.1016/j.etap.2016.01.006
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发表时间：
2016-03-01
影响因子：
4.3
通讯作者：
Cai, Leiming
中科院分区：
文献类型：
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作者：
Jiang, Jinhua;Chen, Yanhong;Cai, Leiming
本研究的目的是调查丙草胺对斑马鱼胚胎发育过程中的毒性作用。结果表明，50、100、200 μg/L丙草胺作用96 h后，斑马鱼下丘脑-垂体-性腺/甲状腺（HPG/HPT）轴相关基因的转录增加，芳香化酶活性、卵黄蛋白原（VTG）和甲状腺激素T3和T4水平也同时上调。丙草胺暴露引起 ROS 水平显着增加，增加抗氧化蛋白（例如 CAT、SOD 和 GPX）的转录和水平。此外，凋亡途径中P53、Mdm2、Bbc3表达以及Caspase3和Caspase9活性的上调表明丙草胺可能引发斑马鱼细胞凋亡。此外，与先天免疫相关的CXCL-C1C、IL-1β和IL-8的转录在丙草胺暴露后下调。这些数据表明，丙草胺可以在斑马鱼胚胎发育过程中同时诱导内分泌干扰、细胞凋亡、氧化应激和免疫毒性。 (C) 2016 年由 Elsevier B.V. 出版
The objectives of the present study were to investigate the toxic effects of pretilachlor on zebrafish during its embryo development. The results demonstrated that the transcription of genes involved in the hypothalamic-pituitary-gonadal/thyroid (HPG/HPT) axis was increased after exposure to 50, 100, 200 mu g/L pretilachlor for 96 h, the aromatase activity, vitellogenin (VTG) and thyroid hormones T3 and T4 levels in zebrafish were also up-regulated simultaneously. Pretilachlor exposure induced a noticeable increase in ROS level, increased the transcription and level of antioxidant proteins (e.g., CAT, SOD and GPX). Moreover, the up-regulation of P53, Mdm2, Bbc3 expression and Caspase3 and Caspase9 activities in the apoptosis pathway suggested pretilachlor might trigger cell apoptosis in zebrafish. In addition, the transcription of CXCL-C1C, IL-1 beta and IL-8 related to the innate immunity was down-regulated after pretilachlor exposure. These data suggested that pretilachlor could simultaneously induce endocrine disruption, apoptosis, oxidative stress and immunotoxicity during zebrafish embryo development. (C) 2016 Published by Elsevier B.V.