Bioglass promotes wound healing through modulating the paracrine effects between macrophages and repairing cells
Bioglass promotes wound healing through modulating the paracrine effects between macrophages and repairing cells
复制标题
生物玻璃通过调节巨噬细胞和修复细胞之间的旁分泌作用来促进伤口愈合
DOI：
10.1039/c7tb01211j
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发表时间：
2017-07-14
影响因子：
7
通讯作者：
Li, Haiyan
中科院分区：
文献类型：
--
作者：
Dong, Xin;Chang, Jiang;Li, Haiyan
炎症反应是创伤愈合过程中的一个关键阶段，巨噬细胞与修复细胞之间的旁分泌作用在创伤愈合中起着关键作用。虽然生物活性玻璃（BG），如45S5玻璃，已被报道能够通过在伤口愈合过程的增殖阶段增强血管生成和胶原沉积来加速皮肤再生，但BG对伤口愈合的炎症反应的影响很少被研究。为了进一步阐明BG促进创面愈合的机制，本研究探讨了BG对巨噬细胞表型转换的影响以及在创面愈合过程中巨噬细胞与修复细胞之间的旁分泌作用。结果显示，与对照培养基相比，BG离子产物将巨噬细胞活化为M2表型，并刺激巨噬细胞表达更多的抗炎和血管生成生长因子。加入BG的巨噬细胞条件培养液可促进内皮细胞和成纤维细胞的迁移，从而增加内皮细胞的毛细血管样网络形成和成纤维细胞的细胞外基质蛋白沉积。当BG粉末应用于大鼠的全层切除伤口时，与对照组相比，BG加速了伤口闭合。在愈合的初始阶段观察到炎症减少，这通过与未进行任何处理的伤口部位相比，在用BG处理的伤口部位中中性粒细胞更少和M2巨噬细胞更多来证明。所有这些结果表明，调节炎症反应是BG促进伤口愈合的关键机制之一。
It has been well accepted that inflammation is a critical stage in the wound healing process and the paracrine effects between macrophages and repairing cells play critical roles in wound healing. Although bioactive glass (BG), such as 45S5 bioglass, has been reported to be able to accelerate skin regeneration through enhancing angiogenesis and collagen deposition in the proliferation stage of the wound healing process, the effects of BG on the inflammation responses of wound healing have rarely been studied. To further elucidate the mechanism of BG enhancing wound healing, in this study, the effects of BG on the phenotype switch of macrophages and paracrine effects between the macrophages and repairing cells in wound healing were investigated. The results showed that BG ionic products activated macrophages towards the M2 phenotype and stimulated macrophages to express more anti-inflammatory and angiogenic growth factors compared to control medium. Conditioned medium of macrophages cultured with BG accelerated the migration of endothelial cells and fibroblasts, thus increasing the capillary-like network formation of endothelial cells and the extracellular matrix protein deposition of fibroblasts. When BG powders were applied to full-thickness excisional wounds of rats, the wound closure was accelerated by BG as compared to the control group. Reduced inflammation during initial stages of healing was observed, which was evidenced by fewer neutrophils and more M2 macrophages in the wound sites treated with BG compared to those without any treatment. All these results indicate that modulating the inflammatory response is one of the critical mechanisms for BG enhancing wound healing.