COMPARATIVE CHEMOTAXONOMIC STUDIES OF MYCOLIC ACID-FREE CORYNEFORM BACTERIA OF HUMAN-ORIGIN
COMPARATIVE CHEMOTAXONOMIC STUDIES OF MYCOLIC ACID-FREE CORYNEFORM BACTERIA OF HUMAN-ORIGIN
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DOI：
10.1128/jcm.31.8.2085-2090.1993
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发表时间：
1993-08-01
影响因子：
9.4
通讯作者：
BIZET, C
中科院分区：
文献类型：
--
作者：
BARREAU, C;BIMET, F;BIZET, C
根据API Coryne系统将42株临床分离株分类为微小棒状杆菌、纹状体棒状杆菌和CDC棒状杆菌I组。通过薄层色谱分析确定了分离物的化学分类学特征。26个分离株被发现有IV型细胞壁（内消旋二氨基庚二酸阿拉伯糖，半乳糖），但不含分枝菌酸。这26个分离株与不含分枝菌酸的参考菌株（包括棒状杆菌NCFB 2768型菌株、“粗糙棒状杆菌”和棒状CDC群12和F2）具有相同的化学分类学特征。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳测定的总蛋白质谱彼此相似，与C. amycolatum型菌株对参比菌株“粗糙棒杆菌”（CIP 100836、CIP80.54、CIP79.37、CIP52.13）、棒杆菌CDC群12和F2（CDC F5771、F5890、G723、G1970）和C. amycolatum密切相关。因此，分枝菌酸阴性菌株与化学型IV壁可能属于一个单一的分类群。DNA杂交研究可以证实这一假设。本研究表明，化学分类学分析的重要性，验证菌株鉴定和完成生化试验的结果，特别是棒状细菌。
Forty-two clinical isolates were classified as Corynebacterium minutissimum, Corynebacterium striatum, and Corynebacterium CDC group I by the API Coryne system. The chemotaxonomic characteristics of the isolates were determined by thin-layer chromatographic analysis. Twenty-six isolates were found to have a type IV cell wall (meso-di-aminopimelic acid arabinose, galactose) but did not contain mycolic acids. These 26 isolates shared chemotaxonomic characteristics with those of mycolic acid-free reference strains (including the Corynebacterium amycolatum NCFB 2768 type strain, ''Corynebacterium asperum,'' and coryneform CDC groups 12 and F2). The total protein profiles of the isolates determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were similar to each other and to that of the C. amycolatum type strain. The profiles of the reference strains ''Corynebacterium asperum'' (CIP 100836, CIP 80.54, CIP 79.37, CIP 52.13), coryneform bacteria CDC groups 12 and F2 (CDC F5771, F5890, G723, G1970), and C. amycolatum were closely related. Thus, the mycolic acid-negative strains with a chemotype IV wall may belong to a single taxon. DNA hybridization studies could confirm this hypothesis. The present study shows the importance of chemotaxonomic analysis for verifying strain identifications and completing results from biochemical tests, particularly for coryneform bacteria.