Towards laser-assisted microfluidic-cell transfection
Towards laser-assisted microfluidic-cell transfection
复制标题
迈向激光辅助微流控细胞转染
DOI：
10.1117/12.2509442
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
K. König
中科院分区：
文献类型：
--
作者：
H. G. Breunig;A. Batista;K. König
通过用聚焦激光脉冲短期照射细胞，可以使细胞膜瞬时渗透。这种“光穿孔”允许外来大分子通过这种原本不可穿透的屏障并进入细胞内部，从而实现高效和“不干涉”的激光辅助细胞转染。我们打算将联合收割机与激光辅助转染技术相结合，通过采用微流体技术来控制通过激光-细胞相互作用区域的细胞流动。精确的流动控制将允许细胞的高通量，并将微流体细胞控制与高效激光辅助转染方案的优点联合收割机相结合。该设置的开发仍在进行中。在这里，概述了背景和主要特点。
By short-term irradiation of a cell with focused laser pulses, the cell membrane can be made transiently permeable. This "optoporation" allows for foreign macro molecules to pass this otherwise impenetrable barrier and enter the cell interior enabling a highly efficient and “hands-off” laser-assisted transfection of cells. We intend to combine a precise flow control of cells with the laser-assisted-transfection technique by employing microfluidics to control the cell flow through the laser-cell interaction region. The precise flow control will allow for a high throughput of cells and combine microfluid cell control with the advantages of the highly efficient laser-assisted transfection scheme. The development of the setup is still in progress. Here, the background and key features are outlined.
影响因子：
4.6
作者：
Georg Breunig H;Uchugonova A;Batista A;König K
通讯作者：
König K