Modification of Fe3O4 magnetic nanoparticles by L-dopa or dopamine as an enzyme support
Modification of Fe3O4 magnetic nanoparticles by L-dopa or dopamine as an enzyme support
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DOI：
10.1007/s11595-006-4480-5
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发表时间：
2008-09
期刊：
影响因子：
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通讯作者：
Hong Peng;Xiao Zhang;Kaixun Huang;Huibi Xu
中科院分区：
文献类型：
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作者：
Hong Peng;Xiao Zhang;Kaixun Huang;Huibi Xu
采用Fe 2+和Fe 3+在氨水中共沉淀的方法制备了Fe 3 O 4磁性纳米粒子，用原子力显微镜测量其粒径约为36 nm。采用超声法分别用左旋多巴和多巴胺对Fe 3 O 4磁性纳米粒子进行修饰。红外光谱分析表明，Fe-O-C键的形成。以戊二醛为偶联剂，以L-多巴和多巴胺为间隔基，将胰蛋白酶（EC：3.4.21.4）直接固定在Fe 3 O 4磁性纳米粒子上。三种磁性纳米粒子的大小和磁性与胰蛋白酶连接前后无明显变化。磁性纳米颗粒上间隔分子的存在，通过增加酶的柔性和改变纳米颗粒表面的微环境，大大提高了结合胰蛋白酶的活性和储存稳定性。
Fe3O4magnetic nanoparticles were prepared by co-precipitation of Fe2+and Fe3+in an ammonia solution, and its size was about 36 nm measured by an atomic force microscope. Fe3O4magnetic nanoparticles were modified by L-dopa or dopamine using sonication method. The analysis of FTIR clearly indicated the formation of Fe-O-C bond. Direct immobilization of trypsin (EC: 3.4.21.4) on Fe3O4magnetic nanoparticles with L-dopa and dopamine spacer was investigated using glutaraldehyde as a coupling agent. No significant changes in the size and magnetic property of the three kinds of magnetic nanoparticles linked with or without trypsin were observed. The existence of the spacer molecule on magnetic nanoparticles could greatly improve the activity and the storage stability of bound trypsin through increasing the flexibility of enzyme and changing the microenvironment on nanoparticles surface compared to the naked magnetic nanoparticles.