EphA2 silencing in nasopharyngeal carcinoma leads to decreased proliferation, invasion and increased sensitization to paclitaxel
EphA2 silencing in nasopharyngeal carcinoma leads to decreased proliferation, invasion and increased sensitization to paclitaxel
复制标题
鼻咽癌中 EphA2 沉默导致增殖、侵袭减少和对紫杉醇的敏感性增加
DOI：
10.3892/ol.2012.746
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发表时间：
2012-09-01
期刊：
影响因子：
2.9
通讯作者：
Tian, Yongquan
中科院分区：
文献类型：
--
作者：
Tan, Pingqing;Liu, Yong;Tian, Yongquan
EphA 2在多种人类癌症中经常过表达和功能改变。然而，其在人鼻咽癌（NPC）中的作用尚不清楚。为探讨EphA 2在鼻咽癌发生发展中的作用，我们采用Western blotting和CCK-8法检测了EphA 2蛋白在鼻咽癌组织中的表达情况。通过体外细胞分选和侵袭实验观察EphA 2抑制剂对EphA 2的影响。结果表明EphA 2在鼻咽癌组织中呈高表达，且EphA 2的表达与鼻咽癌的T分期、临床分期及淋巴结转移密切相关。慢病毒载体介导的EphA 2特异性shRNA感染人鼻咽癌5- 8 F细胞后，可显著抑制5- 8 F细胞的生长、侵袭，并显著增强5- 8 F细胞对紫杉醇的敏感性。总的来说，我们的研究结果表明，EphA 2参与恶性细胞行为，是一个潜在的治疗目标，在人类NPC。
EphA2 is frequently overexpressed and functionally altered in a variety of human cancers. However, its roles in human nasopharyngeal carcinoma (NPC) remain unclear. To investigate the roles of EphA2 in the development and progression of NPC, we initially evaluated the expression pattern of EphA2 protein in NPC tissues using western blotting and CCK-8 assay. Fluorescence-activated cell sorting analysis and invasion assay were conducted to observe the effects of EphA2 inhibition in vivo. Our results demonstrated that EphA2 was overexpressed in NPC specimens and the expression of EphA2 was significantly associated with T classification, advanced clinical stage and lymph node metastasis. Moreover, human NPC 5-8F cells were infected with lentiviral vector-mediated EphA2-specific shRNA, which resulted in the significant inhibition of cell growth, invasion of 5-8F cells and markedly enhanced the sensitivity of 5-8F cells to the chemotherapeutic agent paclitaxel in vitro. Collectively, our results demonstrate that EphA2 is involved in malignant cell behavior and is a potential therapeutic target in human NPC.