Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
Parasite persistence in treated chagasic patients revealed by xenodiagnosis and polymerase chain reaction
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DOI：
10.1590/s0074-02762001000600014
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发表时间：
2001-08-01
影响因子：
2.8
通讯作者：
Fernandes, O
中科院分区：
文献类型：
--
作者：
Britto, C;Silveira, C;Fernandes, O
将聚合酶链反应（PCR）与杀锥虫化疗后20年进行的异种诊断进行比较，以研究寄生虫清除。85名表现出阳性异种诊断的查加斯病血清阳性个体接受了特定药物治疗; 37名处于急性期，48名处于慢性期。15例慢性无症状患者接受安慰剂治疗。急性期治疗导致73%病例的FCR阴性结果，而86%的异种诊断为阴性。在慢性期，65%的患者PCR为阴性，83%导致异种诊断阴性结果。关于未治疗组（安慰剂），73%的异种诊断结果为阴性，其中36%的PCR结果为阳性。认为血清阴性的个体（n=10）在PCR中呈现明确的阴性结果，表明寄生虫DNA消除。17名个体的抗体滴度降低至最终结果被认为是可疑的水平，其中16名呈现阴性PCR。与常规技术相比，分子方法在证明接受化疗的恰加斯病患者的持续感染方面具有明显的优势。
Polymerase chain reaction (PCR) was compared with xenodiagnosis performed 20 years after trypanocidal chemotherapy to investigate parasite clearance. Eighty-five seropositive individuals for Chagas disease presenting a positive xenodiagnosis were treated with specific drugs; 37 in the acute phase and 48 in the chronic phase. Fifteen chronic assymptomatic patients received a placebo. Treatment in the acute phase led to FCR negative results in 73% of the cases, while xenodiagnosis was negative in 86%. In the chronic phase, PCR was negative in 65% of the patients and 83% led to xenodiagnosis negative results. Regarding the untreated group (placebo), 73% gave negative results by xenodiagnosis, of which 36% were positive by PCR. Individuals that were considered seronegative (n=10), presented unequivocally negative results in the PCR demonstrating the elimination of parasite DNA. Seventeen individuals had their antibodies titers decreased to such a level that the final results were considered as doubtful and 16 of them presented negative PCR. The molecular method represents a clear advantage over conventional techniques to demonstrate persistent infections in Chagas disease patients that underwent chemotherapy.