The effect of electroacupuncture on tendon repair in a rat Achilles tendon rupture model
The effect of electroacupuncture on tendon repair in a rat Achilles tendon rupture model
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DOI：
10.1136/acupmed-2014-010611
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发表时间：
2015-02-01
影响因子：
2.5
通讯作者：
Kitakoji, Hiroshi
中科院分区：
文献类型：
--
作者：
Inoue, Motohiro;Nakajima, Miwa;Kitakoji, Hiroshi
目的 通过组织学和力学评价探讨电针（EA）对跟腱断裂大鼠模型术后早期肌腱修复的影响。方法 90只Wistar大鼠制备跟腱断裂模型，随机分为电针组、手法针组和对照组。电针组大鼠自模型制备后第1天起每日接受电针治疗（脉冲宽度5 ms；刺激频率50 Hz；刺激强度20 μA；刺激时间20 min）直至评估日（模型制备后7或10天），此时对感兴趣区域进行取样，采用体外方法评估肌腱修复情况。测量总细胞计数以及转化生长因子-β 1 (TGF-β 1) 和碱性成纤维细胞生长因子 (b-FGF) 染色阳性的细胞数。模型制备后10天进行张力测试，测量修复肌腱的最大断裂强度。结果电针组的细胞总数和b-FGF阳性细胞数均显着较高（p
Objective To examine the effect of electroacupuncture (EA) on early post-rupture tendon repair in a rat model of Achilles tendon rupture using histological and mechanical evaluation.Methods An Achilles tendon rupture model was prepared in 90 Wistar rats, which were randomly assigned to EA, manual acupuncture or control groups. Rats in the EA group received EA (pulse width 5 ms; stimulation frequency 50 Hz; stimulation strength 20 mu A; stimulation time 20 min) daily from 1 day following model preparation until the day of assessment (either 7 or 10 days after model preparation), when the region of interest was sampled to assess tendon repair using in vitro methods. Total cell count and the number of cells staining positive for transforming growth factor-beta 1 (TGF-beta 1) and basic fibroblast growth factor (b-FGF) were measured. Tension tests were performed 10 days after model preparation to measure the maximum breaking strength of the repaired tendon.Results Both the total cell count and the number of cells positive for b-FGF were significantly higher in the EA group (p