VESICULAR STOMATITIS VIRUS-M PROTEIN MAY BE INSIDE THE RIBONUCLEOCAPSID COIL
VESICULAR STOMATITIS VIRUS-M PROTEIN MAY BE INSIDE THE RIBONUCLEOCAPSID COIL
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DOI：
10.1128/jvi.67.12.7246-7253.1993
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发表时间：
1993-12-01
影响因子：
5.4
通讯作者：
RUIGROK, RWH
中科院分区：
文献类型：
--
作者：
BARGE, A;GAUDIN, Y;RUIGROK, RWH
水泡性口炎病毒是一种包膜病毒，具有外部糖蛋白G和核衣壳，其与M蛋白一起形成紧密的螺旋盘绕结构：骨架。对骨骼制备物进行负染色和免疫电镜研究，以确定M蛋白的定位。这些研究产生了弹状病毒结构的新模型，其中核衣壳缠绕在含有M蛋白的核心周围。该模型预测M和脂质之间的接触仅在骨架的最末端，这是通过脂质体结合研究证实的。
Vesicular stomatitis virus is an enveloped virus with an external glycoprotein G and a nucleocapsid that form, together with the M protein, a tight helically coiled structure: the skeleton. Negative staining and immunoelectron microscopy studies on skeleton preparations were performed to determine the localization of the M protein. These studies have resulted in a new model for the structure of rhabdoviruses in which the nucleocapsid is wound around a core containing the M protein. This model predicts contact between M and lipid only at the extreme ends of the skeleton, which is confirmed by skeleton-liposome binding studies.