Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene
Identification of Flatfish Species Using Polymerase Chain Reaction (PCR) Amplification and Restriction Analysis of the Cytochrome b Gene
复制标题
使用聚合酶链反应 (PCR) 扩增和细胞色素 b 基因限制性分析鉴定比目鱼种类
DOI：
10.1111/j.1365-2621.1998.tb15710.x
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发表时间：
2008
影响因子：
3.9
通讯作者：
Rosario Martín
中科院分区：
文献类型：
--
作者：
A. Céspedes;T. García;E. Carrera;I. González;B. Sanz;P. Hernández;Rosario Martín
作者：Anna Cespedes，Teresa GARCIA，Esther Carrera，伊莎贝尔Gonzalez，Bernabe Sanz，巴勃罗E.应用聚合酶链反应（PCR）扩增细胞色素B基因的一个保守区，用限制性酶切位点分析法对比目鱼（Solea solea）、欧洲鲽（Pleuronectes platessa）和比目鱼（Platichthys flusus）进行了特异性鉴定。用Nci Ⅰ、Sau 3AI和Hinf Ⅰ内切酶消化PCR产物，然后进行琼脂糖凝胶电泳，得到特异性图谱，可直接鉴定鱼类。此方法将有助于制定比目鱼种类鉴定的标签法规.关键词：种类，鉴定，比目鱼，PCR，细胞色素B
ANA CESPEDES, TERESA GARCIA, ESTHER CARRERA, ISABEL GONZALEZ,BERNABE SANZ, PABLO E. HERNANDEZ and ROSARIO MARTINABSTRACTRestriction site analysis of PCR products from a conservedregion of the cytochrome b gene has been used for the spe-cific identification of sole (Solea solea), European plaice(Pleuronectes platessa) and flounder (Platichthys flesus).Polymerase chain reaction (PCR) amplification of the cyto-chrome b gene using universal primers produced a 359 bpfragment in all species analyzed. Digestion of the PCR prod-ucts with Nci I, Sau 3AI and Hinf I endonucleases, followed byagarose gel electrophoresis of the digested PCR products,yielded specific profiles that enabled direct identification ofthe fish species. This methodology should prove useful forenforcing labeling regulations in the authentication of flatfishspecies.Key Words: species, identification, flatfish, PCR, cyto-chrome b