A Tri-Channel Oxide Transistor Concept for the Rapid Detection of Biomolecules Including the SARS-CoV-2 Spike Protein
A Tri-Channel Oxide Transistor Concept for the Rapid Detection of Biomolecules Including the SARS-CoV-2 Spike Protein
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DOI：
10.1002/adma.202104608
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发表时间：
2021-11-18
影响因子：
29.4
通讯作者：
Anthopoulos, Thomas D.
中科院分区：
文献类型：
--
作者：
Lin, Yen-Hung;Han, Yang;Anthopoulos, Thomas D.
可以实时检测生物分子的固态晶体管传感器对于新兴的生物分析应用非常有吸引力。然而，将可升级制造与所需性能相结合仍然具有挑战性。这里开发了一种替代生物传感器晶体管概念，它依赖于溶液处理的 In2O3/ZnO 半导体异质结，该异质结具有几何工程三通道架构，可快速、实时检测重要的生物分子。该传感器结合了高电子迁移率通道（归因于 In2O3/ZnO 异质界面的电子特性），该通道紧邻具有束缚分析物受体的传感表面。不寻常的三通道设计能够实现埋藏电子通道与受体-分析物相互作用过程中发生的静电扰动之间的强耦合，从而能够对低至阿托摩尔 (am) 浓度的生物分子进行稳健、实时检测。实验结果得到了广泛的器件模拟的证实，凸显了异质结三通道设计的独特优势。通过使用严重急性呼吸综合征冠状病毒 2 (SARS-CoV-2) 抗体受体对几何工程通道的表面进行功能化，在生理相关条件下，可在 2 分钟内实时检测低至 am 浓度的 SARS-CoV-2 刺突 S1 蛋白。
Solid-state transistor sensors that can detect biomolecules in real time are highly attractive for emerging bioanalytical applications. However, combining upscalable manufacturing with the required performance remains challenging. Here, an alternative biosensor transistor concept is developed, which relies on a solution-processed In2O3/ZnO semiconducting heterojunction featuring a geometrically engineered tri-channel architecture for the rapid, real-time detection of important biomolecules. The sensor combines a high electron mobility channel, attributed to the electronic properties of the In2O3/ZnO heterointerface, in close proximity to a sensing surface featuring tethered analyte receptors. The unusual tri-channel design enables strong coupling between the buried electron channel and electrostatic perturbations occurring during receptor-analyte interactions allowing for robust, real-time detection of biomolecules down to attomolar (am) concentrations. The experimental findings are corroborated by extensive device simulations, highlighting the unique advantages of the heterojunction tri-channel design. By functionalizing the surface of the geometrically engineered channel with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody receptors, real-time detection of the SARS-CoV-2 spike S1 protein down to am concentrations is demonstrated in under 2 min in physiological relevant conditions.