Spontaneous expression of neural phenotype and NGF, TrkA, TrkB genes in marrow stromal cells
Spontaneous expression of neural phenotype and NGF, TrkA, TrkB genes in marrow stromal cells
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骨髓基质细胞中神经表型和 NGF、TrkA、TrkB 基因的自发表达
DOI：
10.1016/j.bbrc.2007.03.013
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发表时间：
2007-05-11
影响因子：
3.1
通讯作者：
Zhao, Huanying
中科院分区：
文献类型：
--
作者：
Li, Na;Yang, Hui;Zhao, Huanying
Marrow stromal cells (MSCs) have the ability to provide growth factors and differentiate into neural-like cells on treating with EGF, bFGF and other factors. We wanted to explore whether growth factors secreted by MSCs itself could induce self-differentiation into neural-like cells. Here, we show that even in the absence of inducing factors, rMSCs spontaneously differentiate into neural-like cells expressing neural markers, such as nestin, beta-tubulin III, Doublecortin (DCX), microtubule-associated protein 2 (MAP2) and neuron-specific enolase (NSE). Furthermore, some cells become neurosphere-like growing in suspension. Compared with control and neural-like rMSCs induced by epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), we found using real-time PCR that self-differentiating rMSCs (SDrMSCs) expressed significantly higher levels of neurotrophic high-affinity receptors (TrkA and TrkB). Coincident with neural marker expression, nerve growth factor (NGF) mRNA was significantly higher than controls despite lower protein levels in the supernatant. Our study suggests that rMSCs have the potential to differentiate into neural cells spontaneously in culture and may contribute towards the natural function of MSCs for neural system in vivo. (c) 2007 Elsevier Inc. All rights reserved.
