Non-invasive determination of metabolite concentrations in human transplanted kidney in vivo by 31P MR spectroscopy
Non-invasive determination of metabolite concentrations in human transplanted kidney in vivo by 31P MR spectroscopy
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DOI：
10.1080/028418500127346054
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发表时间：
2000-11-01
期刊：
影响因子：
1.3
通讯作者：
Lackner, K
中科院分区：
文献类型：
--
作者：
Kugel, H;Wittsack, HJ;Lackner, K
目的：利用表面线圈定量P-31磁共振光谱（MR）技术，研究人体移植肾体内含磷代谢物的浓度，并与已有数据进行比较。材料与方法：在5例功能良好的移植肾患者中，采用表面线圈定量光谱方案，采用三维定位图像选择体内光谱技术获得P-31光谱。通过时域拟合确定的松弛校正信号强度用于推导含磷酸盐代谢物的绝对摩尔浓度。结果：所有光谱中很少或没有磷酸肌酸证实没有肌肉污染，证实了适当的体积定位。移植肾的平均浓度为：ATP 1.60+/-0.26 mmol/1, PDE 2.14+/-0.91 mmol/l, P-i 0.66+/-0.25 mmol/l, PME 2.32+/-0.50 mmol/l。这些值与以前报告的由其他技术确定的值一致。结论：利用MRS无创测定人体肾脏绝对代谢物浓度是对信号强度比检测移植肾脏能量代谢病理变化的补充。
Purpose: To investigate concentrations of phosphorus-containing metabolites in human transplanted kidney in vivo by quantitative P-31 MR spectroscopy (MRS) using surface coils and to compare the obtained values with previous data.Material and Methods: In 5 patients with well functioning transplanted kidneys, P-31 spectra were obtained with the three-dimensional localization image-selected in vivo spectroscopy technique applying a protocol for quantitative spectroscopy using surface coils. Relaxation corrected signal intensities determined by time domain fitting were used to derive absolute molar concentrations for phosphate-containing metabolites.Results: Little or no phosphocreatine in all spectra verified the absence of muscle contamination, confirming proper volume localization. The mean concentrations in the transplanted kidneys were as follows: ATP 1.60+/-0.26 mmol/1, PDE 2.14+/-0.91 mmol/l, P-i 0.66+/-0.25 mmol/l, PME 2.32+/-0.50 mmol/l. These values are consistent with previously reported values determined by other techniques.Conclusion: The non-invasive determination of absolute metabolite concentrations in human kidney using MRS supplements the use of signal intensity ratios to detect pathologic changes in the energy metabolism of transplanted kidneys.