The effect of oocyte size and bitch age upon oocyte nuclear maturation in vitro
The effect of oocyte size and bitch age upon oocyte nuclear maturation in vitro
复制标题
DOI：
10.1016/s0093-691x(98)00044-2
复制
发表时间：
1998-04-01
期刊：
影响因子：
2.8
通讯作者：
England, GCW
中科院分区：
文献类型：
--
作者：
Hewitt, DA;England, GCW
母犬的体内成熟尚未得到充分的研究，该技术的完善对于濒危犬种未来的配子挽救计划至关重要。为了使这些技术获得最佳成功，了解个体动物和卵母细胞对成熟能力的影响将是有用的。因此，两个因素进行了研究，使用乙酰地衣红染色技术，这已被证明是有效的远监测犬卵母细胞的核成熟后，卵母细胞培养在培养基中补充牛血清白蛋白（BSA）。体外培养不同大小的卵母细胞并监测其核成熟。结果表明，选择通过卵泡内生长获得减数分裂能力的卵母细胞是体外成熟的重要条件。然后比较了1至6岁母犬和7岁及以上母犬卵母细胞的体外成熟，发现年轻母犬的卵母细胞比从老年动物收集的卵母细胞成熟的潜力更大。(C)1998年，Elsevier Science Inc.
In vivo maturation in the bitch has yet to be fully investigated, and perfection of the technique is essential for future gamete salvage programs in endangered canine species. For optimal success with these techniques, knowledge of the individual animal and of oocyte effects upon maturational competence would be useful. Two factors were therefore studied using an aceto-orcein staining technique, which has been shown to be effective far monitoring nuclear maturation of canine oocytes following oocyte culture in medium supplemented with bovine serum albumin (BSA). Oocytes of different sizes were cultured in vitro and their nuclear maturation monitored. It was shown that the selection of oocytes which had acquired meiotic competence through adequate intrafollicular growth was important for in vitro maturation. In vitro maturation of oocytes from bitches aged 1 to 6 yr, and from those 7 yr and older was then compared, and it was found that oocytes from young bitches had a greater potential to mature than those collected from the older animals. (C) 1998 by Elsevier Science Inc.